ASE法提取飼料中三聚氰胺的研究進(jìn)展

生物工程論文編號:SW054  論文字?jǐn)?shù):10289,頁數(shù):27

目  錄
中文摘要 I
英文摘要 II
目錄 III
1. 緒論 1
 1.1  引言 1
 1.2  三聚氰胺的性質(zhì)與毒性 1
 1.3  飼料中三聚氰胺的來源 2
 1.4  三聚氰胺的前處理 3
 1.5  飼料中三聚氰胺的分析方法 3
 2. 實驗部分 6
 2.1 試劑與儀器 6
 2.2 材料 7
 2.3 方法 7
 2.4 ASE的提取條件確定 8
 2.5 色譜條件 8
 2.6 標(biāo)準(zhǔn)曲線的繪制 8
 2.7 加標(biāo)回收 9
 2.8 ASE法與超聲波法比較 9
 2.9 結(jié)果計算 9
 2.10 本章小結(jié) 9
3. 結(jié)果與討論 10
 3.1 ASE的提取條件 10
 3.2 色譜條件的優(yōu)化 13
 3.3 方法線性 14
 3.4 ASE萃取法優(yōu)于超聲波法的原因分析 17
 3.5 本章小結(jié) 17
4．總結(jié)與展望 19
 4.1 ASE法提取飼料中三聚氰胺的建立 19
 4.2 ASE法提取飼料中三聚氰胺的操作要點 19
 4.3 飼料中三聚氰胺的含量測定及意義 19
 4.4 問題及展望 19
致謝 21
參考文獻(xiàn) 22

摘要：三聚氰胺是一種三嗪類含氮雜環(huán)有機(jī)化合物。 由于其較高的含氮量而被添加于食品和飼料中充當(dāng)?shù)鞍踪|(zhì)含量，對動物和人體造成了一定的危害。本文闡訴了使用ASE快速萃取技術(shù)來提取飼料中的三聚氰胺，并與超聲波提取法做比較，最后使用氣相色譜-質(zhì)譜聯(lián)用（GC-MS）檢測法檢測比較結(jié)果。本方法從濃度1.25µg/ml到20.0µg/ml范圍內(nèi)線性關(guān)系良好，線性方程為y=490514x+19766，相關(guān)系數(shù)R=0.9994，檢測限為0.05 mg/kg，通過空白樣品飼料外標(biāo)法加標(biāo)，回收率為96%。采用本方法對含有三聚氰胺的陽性樣品進(jìn)行4次平行檢測，測定結(jié)果為19.50 mg/kg，RSD為2.68 %，采用超聲波法-GC/MS法進(jìn)行同一樣品的4次平行檢測，平均值為14.65mg/kg，RSD為2.79 %。ASE法萃取效率明顯優(yōu)于超聲萃取法。
關(guān)鍵詞：三聚氰胺；快速溶劑萃取（ASE）；飼料；氣相色譜質(zhì)譜

The research progress of determination the melamine in feed by ASE method
Abstract：Melamine is a nitrogen-containing heterocyclic triazine compounds. .Due to its rich in nitrogen, it has been added to give a false impression of high protein content. Melamine added to food and animal feeds could possibly cause fatal kidney stones. Determination of melamine is therefore of large importance to ensure food safety. This article reviewed a new method of ASE v. rapid extraction technology to extract the melamine in feed and sediments of different conditions and with the conventional method of extraction methods for comparison, the last use of GC-MS assay test results of the comparison. A standard curve was obtained and the regression equation was y=490514x+19766,correlation coefficient R=0.9994,the detection limit was 0.05 mg/kg,the recoveries of feed blank sample were 96% by external standard calibration.Using this method to detect melamine-positive samples for four times in parallel,measurement results were 19.50 mg/kg, RSD was 2.68 %.Using the method of Ultrasonic extraction-GC/MS to detect the same melamine-positive samples for four times in parallel,measurement results were 14.65 mg/kg, RSD was 2.79 %.The extraction efficiency of ASE method obtained was better than Ultrasonic extraction.
Keywords: melamine; accelerated solvent extraction; feed; GC/MS