The Methond of Saving Clen-BSA and Rapid Detection to Clenbuterol Hydrochloride in Urine Sample
Abstract: Diazotization method using small molecule hapten Clenbuterol(Clen) coupled to bovine serum albumin(BSA) on the system was completely anti-clenbuterol(Clen-BSA).Clen-BSA GICA in law used to T line,which contains a protein component,so vulnerable to the effects of external factors changed through the addition of protective agent and control of temperature,research to determine the best protective agent.The use of immune competition law,will polyclonal anti-clenbuterol antibody-colloidal gold complexes in the colloidal gold-coated mat combination,at the same time,Clen-BSA coated nitrocellulose membrane in the surface as a test line(T line),will be polyclonal anti-clenbuterol antibody goat anti-mouse antibody-coated cellulose nitrate film in the surface as a quality control lines(C lines).T line of artificial antigen and sample competition in the combination of clenbuterol colloidal gold labeled polyclonal antibody Clenbuterol by T line and C line of color contrast to the results read out.Using the detection reagent sample testing organization,the sensitivity of the minimum value of up to 5ppb,the entire testing process takes 3-5min,and with Ractopamine,sulfadoxine-methylisothiourea Tebuconazole,terbutaline without cross-reaction.Detection reagent with high sensitivity and specificity,operation convenient,stable and reliable organization in the hydrochloric acid can be used as the scene Karen residues means of monitoring the effective screening.
Keywords：Clenbuterol; bovine serum albumin; preservation; colloidal gold marker; immunochromatography assay
