城市中水綜合利用工程中高效異養(yǎng)硝化菌的篩選

生物工程論文編號(hào):SW077  論文字?jǐn)?shù):9731,頁(yè)數(shù):22

 目錄
中文摘要： I
英文摘要: II
1  緒論 1
1.1引言 1
1.2廢水生物脫氮工藝的發(fā)展 3
 1.3 硝化菌的生長(zhǎng)周期 6
1.4異養(yǎng)硝化菌研究存在的問題 8
2. 實(shí)驗(yàn)部分 8
 2.1 材料與方法 8
 2.2 硝化能力的測(cè)定 10
2.3 分析方法和儀器 10
3 結(jié)果與討論 14
 3.1 異養(yǎng)硝化菌的獲得及其菌落形態(tài) 14
 3.2 硝化能力的測(cè)定 14
3.3 溫度對(duì)硝化速率的影響 15
4 展望 17
致  謝 18
參考文獻(xiàn) 19

摘 要：目前水資源問題越趨嚴(yán)重，而將城市中水綜合利用是緩解水資源緊張的有效途徑，妨礙中水循環(huán)利用的主要問題之一是氨氮超標(biāo)，因此如何將城市污水處理廠出水進(jìn)行深度脫氮成為研究的熱點(diǎn)。傳統(tǒng)的硝化菌多數(shù)是自養(yǎng)型的，在工程應(yīng)用上存在缺陷。為獲得更具工程應(yīng)用價(jià)值的異養(yǎng)硝化菌，對(duì)來自西密湖底污泥中的異養(yǎng)硝化菌進(jìn)行富集、篩選及分離純化。實(shí)驗(yàn)分離得到2株菌種，通過納氏分光光度法測(cè)定樣品中的氨氮和N-(1-萘基)-乙二胺光度法測(cè)定亞硝基氮的濃度來鑒定其硝化能力，并做了溫度對(duì)其硝化速率的影響，結(jié)果表明在溫度為25-30℃時(shí)，硝化速率達(dá)到最大。

關(guān)鍵詞：異養(yǎng)硝化菌；篩選；納氏分光光度；硝化速率

 The screening of  efficiently Nitrifying bacteria in the comprehensive utilization of urban water

Abstract: Now the serious problems of water. The urban water utilization of water resources is the effective way to relieve  the tension. Ammonia nitrogen is one of the main problems who block water recycling. Therefore, how to urban sewage treatment plants will water depth denitrification become the research focus This article introduced the screening domestication and purification of the Nitrifying bacteria from ximi lake. There are two strains isolated from experiment By degrees spectrophotometry samples of ammonia nitrogen and N - (1 - naphthalene) - ethylenediamine spectrophotometric determination and concentration of nitrogen nitro identifying its nitrification ability. And the temperature of the influence of nitration rate, the results indicate that the temperature of 25-30 ° c, the maximum rate of nitration.

Keywords：Heterotrophic nitrification, screening, Purification, Leonard's spectrophotometry, Nitrifying rate