蛋白制品中三聚氰胺及氰尿酸含量的檢測方法研究

生物工程論文編號:SW079  論文字數:8436,頁數:26

目  錄
中文摘要 I
英文摘要 II
目錄 III
1. 緒論 1
1.1  三聚氰胺及氰尿酸簡介 1
1.2  三聚氰胺的毒性 2 
1.3  三聚氰胺的檢測方法 3
1.4  高效液相色譜法簡介 3
2.實驗部分 4
2.1  實驗材料 4
 2.2  樣品處理 5
 2.3  溶液的配制 5
 2.4  實驗方法 6
 2.5 標準曲線 6
 2.6 樣品含量測定 8
3．結果與討論 8
 3.1 三聚氰胺檢測結果與討論    8
 3.2 氰尿酸檢測結果與討論 17
4．總結與展望 20
致謝 21
參考文獻 22

摘 要：建立了高效液相色譜法檢測乳制品中三聚氰胺及氰尿酸的方法。樣品由1%三氯乙酸-乙腈超聲提取，加入蛋白沉淀劑進行純化，濾液經0.45m膜后進樣分析。三聚氰胺的色譜條件及結果：色譜柱ZORBAX SB-Aq（250mm×4.6mm，5m），流動相為磷酸-三乙胺溶液（pH=3），流速為0.6ml/min，檢測波長為240nm，回收率為101.7～124.0%，相對標準偏差在3.5～3.9%。氰尿酸的色譜條件及結果：色譜柱ZORBAX SB-Aq（250mm×4.6mm，5m），流動相為磷酸-三乙胺溶液（pH=3），流速為0.6ml/min，檢測波長為225nm。采用HPLC方法測定蛋白制品中三聚氰胺及氰尿酸的含量具有高效、快速、準確、樣品浪費少等等優點。適合企業及檢測部門使用。
關鍵詞：三聚氰胺；氰尿酸；高效液相色譜（HPLC）；蛋白制品

Detection Method of Melamine and Cyanuric acid in the protein products

Abstract: Established a high-performance liquid chromatography detection of dairy products of melamine and cyanuric acid method. Samples were extracted by blending with 10% TCA and acetonitrile. The extract was purified by precipitating protein. Filtrated 0.45m membrane. The HPLC conditions of melamine: ZORBAX SB-Aq（250mm×4.6mm, 5m）, phosphoric acid-triethylamine as mobile phase and UV detection at 240nm. The average recoveries were 101.7∼124.0% and RSD was 3.5∼3.9%.The HPLC conditions of Cyanuric acid: ZORBAX SB-Aq（250mm×4.6mm, 5m）, phosphoric acid-triethylamine as mobile phase and UV detection at 225nm. HPLC method which was using in the determination of protein products of melamine and cyanuric acid ,it had advantage like high efficiency, rapid, accurate and so on. It is suitable for detection of enterprises and departments.
Keywords：melamine ;cyanuric acid ;High Performance Liquid Chromatography (HPLC); protein products