HPLC法測定水產品中阿苯達唑及其主要代謝物殘留的研究

生物工程論文編號:SW080  論文字數:10084,頁數:28

目  錄
中文摘要 I
英文摘要 II
目錄 III
1. 緒論 1
 1.1  前言 1
 1.2 阿苯達唑及其主要代謝物檢測技術的國內外發展現狀 2
2. 實驗部分 5
 2.1  試劑和材料 5
 2.2  儀器設備 5
 2.3  實驗操作方法 6
3. 結果與討論 8
 3.1   樣品前處理條件的選擇 8
 3.2   色譜條件的選擇 8
 3.3   精密度和準確度 14
 3.4   方法的靈敏度和檢測限 19
4．總結與展望 20

致謝 21
參考文獻 22

摘 要：本文主要闡述了應用高效液相色譜（HPLC）方法測定水產品中阿苯達唑（ABZ）及其兩種主要代謝物砜（ABZSO2）、亞砜（ABZSO）殘留量的研究。色譜條件是：色譜柱為ZORBAX-SB 5μm C18柱；流動相是酸化乙腈：水(25:75；v/v)；甲醇-磷酸緩沖液（75︰25；v/v），流速是1.0mL/min，柱溫是30℃，選擇的檢測波長是295.4nm。采用甲醇：磷酸緩沖液=75：25定容系列標準液，阿苯達唑、阿苯達唑砜、阿苯達唑亞砜在0.1～10μg/mL范圍內線性關系良好，加標濃度在100μg/kg～500μg/kg時，回收率分別ABZSO為83%～95%；ABZSO2為85%～97%；ABZ為70%～80%。研究結果顯示，該方法的最低檢測限、準確度和精密度均符合殘留檢測方法的要求。此檢測分析方法處理樣品快速、高效、準確，可作為水產品中阿苯達唑及其主要代謝物的有效檢測方法。
關鍵詞：水產品、高效液相色譜法、阿苯達唑及其代謝物

Determination of Albendazole and its metabolites in Aquatic products by HPLC
Abstract: The determination of Albendazole and its metabolites in aquatic products was established by using high performance liquid chromatography (Agilent-1100, USA) with a photodiode array detector detector (DAD, USA), a C18 column (ZORBAX-SB). The mobile phase was a mixture of acidified acetonitrile and water (25/75, v/v) and a mixture of methanol and phosphate buffer (75/25, v/v). 20 µl samples at room temperature injected onto the column through a 20 µl sample loop. Column temperature is 300C.The mobile phase rate was delivered at 1.0 ml/min. Detection wavelength: 295.4nm. In the range of 0. 1 - 10μg/ml, the results of Albendazole and its metabolite are linearly dependent. The mean extraction recoveries of Albendazole and its metabolites ranged from 80% to 90%. The method of determining the Albendazole and its metabolites in aquatic product was established by using reversed-phase HPLC, which had characters of simple and rapid operation, high yield and good reproducibility.

Keywords：Aquatic products, high performance liquid chromatography, Albendazole and its metabolites