甘薯抗氧化性及其黃酮含量的基因型差異研究

生物工程論文編號:SW096  論文字數:14532,頁數:29

目  錄
中文摘要 I
英文摘要 II
目  錄 III
1. 緒 論 1
1.1引言 1
1.2 甘薯抗氧化性研究 3
1.3 本章小結 7
2. 實驗部分 8
2.1 試劑、儀器與實驗材料 8
2.2 實驗設計和樣品處理 10
2.3 本章小結 12

3. 結果與討論 13
3.1 總抗氧化能力測定 13
3.2 甘薯黃酮含量測定 14
3.3 甘薯黃酮的NIRS分析 17
3.4 甘薯抗氧化能力與黃酮含量的相關性研究 20
3.5 本章小結 21
4. 總結與展望 22
致  謝 23
參考文獻 24

 摘 要：甘薯具有一定的營養價值和藥用功能，它能消除活性氧，具有抗氧化能力。本文著重研究甘薯總抗氧化物質及黃酮類物質的提取和測定方法，進而分析基因型間的差異。由此了解其抗氧化的程度，幫助人們合理利用食品營養、研制能夠預防癌癥及心血管等方面疾病的功能性食品，還為甘薯黃酮類物質的開發和高黃酮高抗氧化性資源的篩選提供依據，促進農副產品的開發利用。實驗表明，甘薯對自由基DPPH有很高的清除能力，所采用的21種基因型甘薯中，對DPPH的清除率最高達到97.3%，17種甘薯對其抑制率在89%以上，最低的為51.4%。采用以蘆丁為標樣的比色法對蕭山，玉環兩個試點的42種甘薯進行黃酮含量的測定，并采用近紅外光譜分析技術得到另4個試點的84種甘薯中黃酮的含量，經分析表明黃酮含量存在顯著的品中間和產地間的差異，紫883，徐55-2，金山1222 等品種穩定性強，受環境影響小，黃酮含量平均值都較高，是良好的選育品種。研究還表明甘薯抗氧化能力與黃酮含量之間為顯著相關。

關鍵詞： 抗氧化性；黃酮；近紅外分析；基因型差異；甘薯

Study on genotypic variation of antioxidant activity
and flavonoid content in sweet potato storage roots

Abstract: Sweet potato has much physiological functionality, which contains rich of nutrition. It has antioxidant activity that can eliminate active oxygen. Extraction and mensuration of total antioxidant and flavonoid were studied in the paper, and genotypic variation was analyzed,which helped people to understand the degree of antioxidant, to reasonably use food nutrition and to develop research on the functional foods to prevent cancer and cardiovascular diseases. It also provides the basis for flavonoids exploiture and the high oxidation resistance and high-flavonoid resource selection, and promote the development and use of agricultural and sideline products. The result showed that sweetpotato had high ability to scavenge DPPH radical. Among 21 genotypes , the scavenging rate on DPPH radicals ranged from 51.4%-97.3%, about 17genotypes exceed 89%. The flavonoid content of sweetpotato has been measured by using Rutin as standard sample. As a result，there was variation in genotypic and location in the flavonoid contents, the Zi 883, Xu 55-2, Jinshan 1222 showed to be the choiceness genotypes, for they have high stabilization to location and high flavonoid content. During the experiment NRIS analysis had been used. The result also showed that the flavonoid content had significantly correlated to antioxidant ability.
Keywords：Antioxidant activity;  Flavonoid;  NIRS analysis;  Genotypic variation; 
Sweet potato