發(fā)酵法生產(chǎn)X7的工藝研究

生物工程論文編號(hào):SW100  論文字?jǐn)?shù):12288,頁(yè)數(shù):30

目  錄
中文摘要 I
英文摘要 II
目錄 III
1.  緒論 1
 1.1  X7的性質(zhì) 1
 1.2  X7的功用 2
 1.3  X7的開(kāi)發(fā) 3
2.  實(shí)驗(yàn)部分 5
 2.1  實(shí)驗(yàn)器材 5
 2.2  實(shí)驗(yàn)方法 6
3.  結(jié)果與討論 8
 3.1  種子配方及種齡種量實(shí)驗(yàn) 8
 3.2  pH、P、Ca實(shí)驗(yàn) 9
 3.3  C源調(diào)整實(shí)驗(yàn) 11
 3.4  N源調(diào)整實(shí)驗(yàn) 14
 3.5  無(wú)機(jī)鹽實(shí)驗(yàn) 16

4.  總結(jié)與展望 19
致謝 20
參考文獻(xiàn) 21

摘　要：X7是一種具有極強(qiáng)的抗氧化性的酮式類(lèi)胡蘿卜素 ,在醫(yī)藥 ,食品 ,化妝品等方面有著極廣闊的應(yīng)用前景。目前,生物技術(shù)生產(chǎn)X7的兩種主要方法:藻類(lèi)培養(yǎng)法和酵母發(fā)酵法。
 A酵母是X7生產(chǎn)中最具潛力的生物來(lái)源。根據(jù)A酵母發(fā)酵工藝原理, 本實(shí)驗(yàn)對(duì)A酵母菌株產(chǎn)生X7的種子培養(yǎng)條件和發(fā)酵培養(yǎng)條件進(jìn)行了初步研究, 并主要對(duì)發(fā)酵過(guò)程作了動(dòng)態(tài)分析. 結(jié)果表明: 種子培養(yǎng)基組成、發(fā)酵培養(yǎng)基組成以及初始pH值、搖瓶裝量與培養(yǎng)時(shí)間和溫度等, 均對(duì)該菌細(xì)胞的X7產(chǎn)量有影響。
 由此，對(duì)A酵母發(fā)酵生產(chǎn)X7的種子培養(yǎng)和發(fā)酵條件包括：種子配方，種齡種量，發(fā)酵配方等進(jìn)行優(yōu)化,得到最佳培養(yǎng)條件。在此條件下發(fā)酵24 h和28 h ,得到比優(yōu)化前高幾倍的X7效價(jià)。達(dá)到優(yōu)化工藝條件、提高生產(chǎn)效率的目的。
 關(guān)鍵詞： X7； A酵母； 發(fā)酵； 優(yōu)化

The handicraft studies of the zymotechnics producing of X7

Abstract: X7 is a kind keto form carotenoid which has strong inoxidability. It has the extremely broad application in the medicine, food, and cosmetics. At present, there are two main methods to produce X7: algal culture and yeast fermentation.
 The A yeast is the most potential biological origin in the X7 production. According to the craft principle of A yeast fermentation, the experiment has carried out a pilot study. And the study reffers to the condition in A yeast culture and mainly in A yeast fermentation. The results indicated that, all of the following has an effect on X7 production. They are: the culture medium of culture and fermentation, initial pH, filling level, as well as raise time and temperature.
 From this, the experiment has been carried on to optimize the condition in A yeast culture and fermentation. It concludes the culture medium of culture and fermentation, the vaccination quantity, raise time, raise quantity, initial pH and so on. Then it obtains the best culture condition. By fermenting for 24 h and 28 h under this condition, the yeast obtains a several times bigger yield of X7, which is compared to before the optimization. So the goal of the optimization of process conditions, and the enhance of the yield is achieved.
Key words: X7; A yeast; Fermentation; Optimization