板栗殼多糖的純化及組成分析

生物工程論文編號(hào):SW128  論文字?jǐn)?shù):7475,頁(yè)數(shù):22

目 錄
中文摘要...............................................................I
英文摘要...............................................................Ⅱ
目 錄..................................................................Ⅲ
1 緒 論................................................................1
1.1 板栗殼概述....................................................1
1.2 板栗殼研究現(xiàn)狀................................................1
1.3 多糖概述.................................................... 2
1.4 多糖研究現(xiàn)狀.................................................2
1.5 本章小結(jié).....................................................3
2 實(shí)驗(yàn)部分 ...........................................................5
2.1 材料與設(shè)備...................................................5
2.2 實(shí)驗(yàn)主要試劑.................................................6
2.3 實(shí)驗(yàn)方法.....................................................6
3 結(jié)果和討論..........................................................10
3.1 板栗殼粗多糖提取.............................................10
3.2 脫蛋白處理...................................................10
 3.3 板栗殼粗多糖的紫外光譜檢測(cè)................................11
3.4 板栗殼粗多糖的分離純化........................................11
3.5 板栗殼多糖的組成分析..........................................13
4 總結(jié)與展望...........................................................17
致 謝..................................................................18
參考文獻(xiàn)...............................................................19

摘要：本實(shí)驗(yàn)以227g板栗殼為原料，用水煮醇沉法提取了板栗殼粗多糖，得到的粗糖蛋白用酶+Sevag法脫蛋白，處理了15次后無(wú)明顯的蛋白層，得到3.7g板栗殼粗多糖，提取率為1.6%；紫外光譜檢測(cè)脫蛋白后的板栗殼粗多糖，顯示在280nm處已無(wú)吸收峰，說(shuō)明蛋白質(zhì)已經(jīng)基本除盡。用Sephacryl 100 HR層析柱對(duì)板栗殼粗多糖進(jìn)行了分離純化,經(jīng)蒸餾水和0.1mol/LNaCl溶液分別洗脫后得到三個(gè)組分cnps1，cnps2，cnps3。將板栗殼粗多糖進(jìn)行酸水解，衍生化處理后，經(jīng)氣-質(zhì)聯(lián)用儀分析得到該粗多糖中由D-木糖，D-甘露糖，D-葡萄糖和D-半乳糖組成。
關(guān)鍵詞：板栗殼；多糖；提取；分離純化；組成分析

Component of and purification on the chestnut chitosan

Abstract: In this study, the Castanea mollissima227g shell was extracted with water and Castanea mollissima shell crude polysaccharide. The crude polysaccharide was deproteined with enzyme and Sevag, after handling 15 times there was no obvious protein layer left.Have Polysaccharides3.7g, extraction rate 1.6%;UV detection showed that there was no absorption peak at 280 nm, that is to say ,the protein in the crude polysaccharide had been removed entirely. 
 Using the Sephacryl 100HR column to purify chestnut shell crude polysacchari-
des,with the mobile phase distilled water and 0.1mol/LNaCl. By the distilled water and 0.1mol/LNaCl solution clear get three components are cnps1, cnps2, cnps3 Polysaccharide chestnut shell rough to acid hydrolysis, derivative then by the GC-MS have D-xylose, D-mannose, D-glucose and D-galactose composition of form analysis of the polysaccharides.
Key Words：Castanea mollissima shell; Polysaccharides; Extraction; Separation and Purification; Composition of