內(nèi)生真菌HCCB3616的分類鑒定

生物工程論文編號(hào):SW141  論文字?jǐn)?shù):9304,頁(yè)數(shù):25

目  錄
中文摘要 I
英文摘要 II
目錄 III
1. 緒論 1
 1.1  植物內(nèi)生菌概況 1
 1.2 PCR原理 4 
 1.3瓊脂糖凝膠電泳原理 5
 1.4立題依據(jù) 5
2. 實(shí)驗(yàn)部分 7
 2.1試驗(yàn)材料 7
 2.2試驗(yàn)方法 8
3.結(jié)果與討論 12
 3.1菌株HCCB3616的生長(zhǎng)速度考察 12
 3.2菌株的形態(tài)、培養(yǎng)特征 13
 3.3 HCCB3616染色體DNA的制備及18S rDNA序列的PCR 15
 3.4 菌株HCCB3616的系統(tǒng)發(fā)育分析及鑒定結(jié)果 14
4．總結(jié)與展望 17
 4.1總結(jié) 17
 4.2.展望 17
致謝 19
參考文獻(xiàn) 20

摘 要： 本論文利用上海來益生物藥物研發(fā)中心的植物內(nèi)生菌資源，通過抗菌模型的篩選，獲得多株活性功能菌株，對(duì)其中一株活性較高的HCCB3616進(jìn)行了菌種鑒定。主要研究工作包括以下幾個(gè)方面：
 對(duì)內(nèi)生真菌HCCB3616進(jìn)行分離和培養(yǎng)，觀察菌體的形態(tài)特征。提取它的染色體DNA，進(jìn)行PCR擴(kuò)增，以確定其種屬分類地位。
 本文采用18S rDNA序列測(cè)定與傳統(tǒng)形態(tài)學(xué)觀察相結(jié)合的方法，對(duì)活性菌株HCCB3616進(jìn)行分類鑒定，各種指標(biāo)鑒定其為鏈格孢霉屬（Alternaria sp.）。
 
關(guān)鍵詞: 內(nèi)生真菌；抗菌；篩選；18S rDNA；鑒定

Assort and identification of the Endophytic Fungus HCCB3616

Abstract:  In this research work, from a large amount of endophytic fungi which were conserved in Shanghai Health Creation Center for Biopharmaceutical R&D had been screened based on the anti-bacterial model, dozens of strains showed activities. The strain HCCB3616 was taxonomically identified. The research work was as follows:
 Isolate and culture the endophytic fungi HCCB3616,then observe its morphological characteristics. Extract its Chromosomal DNA for PCR amplification to determine its taxonomic status of species.
 By using the methods of 18S rDNA sequences determination combined with morphological observation, the endophytic fungus HCCB3616 was identified as Alternaria sp.
 
Keywords：Endophytic Fungi; Anti-bacterial; 18S rDNA; Identification