枯草芽孢桿菌微生態制劑的研制

生物工程論文編號:SW191  論文字數:13863,頁數:27

目  錄
中文摘要 I
英文摘要 II
目錄 III
1. 緒論 1
 1.1前言 1
 1.2動物微生態制劑的研究 1
 1.2.1動物微生態制劑的作用機理 1
  1.2.2芽孢桿菌類微生態制劑及種類 2
 1.2.3枯草芽孢桿菌的生物學性質與作用機理 2
 1.2.4枯草芽孢桿菌的作用機理 2
  1.2.5發酵豆粉的優點 3
   1.2.6農業部飼料工業中心研究表明 4
 1.2.6.1此制劑可降低豬肉抗生素殘留 4
  1.3 本章小結 4
2. 實驗部分 6
2.1試驗材料和儀器 6
 2.1.1材料 6
 2.1.2儀器 6
 2.1.3培養基 6
2.2試驗方法 7
 2.2.1實驗室操作要點及工藝流程 7
 2.2.2工業化大型生產工藝流程 7
 2.2.3培養方法 7
 2.2.4菌體生物量的測定 7
 2.2.5枯草芽孢桿菌菌懸液的菌密度測定 8
  2.2.6菌種的載體添加 8
 2.2.7真空干燥 8
 2.2.8研磨粉碎 8
 2.2.9保存 8
2.3制作發酵豆粕 9
 2.3.1準備物品 9
 2.3.2發酵豆粉 9
2.4微生態制劑在豬飼養中的應用 10
 2.4.1試驗地點 10
 2.4.2試驗時間 10
2.5 本章小結 10
3. 結果與討論 11
3.1單因素法培養條件的優化 11
 3.1.1溫度對發酵菌數的影響 11
 3.1.2初始pH值對發酵菌數的影響 11
 3.1.3種量對發酵菌數的影響 11
3.2菌種的固定 11
3.3菌落計數 12
3.4固定化后的海藻酸鈉菌固體的干燥 12
 3.4.1步驟1 12
 3.4.2步驟2 12
3.5發酵豆粉成份分析 13
3.6試驗動物分組及處理設置 13
 3.6.1結果討論 13
  3.6.2研究產品在三個養豬場的試驗結果 16
 3.6.3添加枯草芽孢桿菌微生態制劑對環境的作用 16
3.7 本章小結 16
4．總結與展望 18
4.1總結 18
4.2展望 18
致謝 21
參考文獻 22

 摘要：微生態制劑是一類新型活菌制劑，合理運用于畜牧生產中可提高畜禽的生產性能。提高機體免疫力，改善畜產品品質，減少環境污染，為一類新型綠色飼料添加劑。本文論述了微生態制劑的作用、機理及采用枯草芽孢桿菌(Bacillus subtilis)的液體發酵工藝，確定枯草芽孢桿菌的最適發酵條件為：發酵溫度30℃，初始pH值7．2，最適接種量5％，最佳干燥溫度為35℃，并以該枯草芽孢桿菌為菌種發酵豆粕配制斷奶仔豬日糧，研究其對斷奶仔豬生產性能及斷奶應激反應的影響，確定發酵豆粕的適宜用量，并評估其經濟效益，為大規模工業化發酵培養和枯草芽孢桿菌微生態制劑的生產的提供了有益借鑒。
關鍵字：微生態制劑；正常菌群；發酵豆粕；飼料添加劑

Abstract : Probiotics is a new type Ecologcs, that the livestock and poultry production performance could be improved when Reasonable application. Which Enhance the body's immune system, improve the quality of animal products and reduce environmental pollution, is a new type of green feed additives.now, the role of probiotics. mechanism and the use of Bacillus subtilis of liquid fermentation is discussed in This paper.Than the optimal conditions for fermentation of Bacillus subtilis was determined: 30°C is a fermentation temperature, 7.2 is the initial pH, 5% is the optimum inoculum, and the 35°C is the best drying temperature. And the fermentation of Bacillus subtilis bacterium is Prepared for a weaning of piglets’ soybean meal, which was to the Research of impact on production performance and stressed weaning reaction on weaned piglets.than the appropriate dosage of fermented soybean meal was determined. And its economic benefits was Assessmented, which is a useful references to a large-scale industrialization fermentation and the production of Bacillus subtilis probiotics.
 
Keywords : probiotics; Normal flora; Fermented soybean meal; Feed Additive