采用生物指示劑對(duì)臭氧消毒效果探討

采用生物指示劑對(duì)臭氧消毒效果探討

摘 要

臭氧消毒滅菌技術(shù)以其消毒效果好、使用方便、對(duì)作業(yè)人員和作業(yè)場(chǎng)所沒(méi)有危害，現(xiàn)在為制藥行業(yè)廣泛采用，主要用于在十萬(wàn)級(jí)條件下臭氧對(duì)環(huán)境的消毒、滅菌。在微生物挑戰(zhàn)性實(shí)驗(yàn)中，傳統(tǒng)的檢驗(yàn)臭氧消毒效果是用枯草芽孢桿菌來(lái)檢測(cè)，但實(shí)驗(yàn)證明枯草芽孢桿菌作為檢測(cè)的指示劑存在著較多的缺點(diǎn)，其殺菌率達(dá)不到微生物挑戰(zhàn)性實(shí)驗(yàn)的標(biāo)準(zhǔn)要求。

為提高檢測(cè)的靈敏度，本實(shí)驗(yàn)分別考察以金黃色葡萄球菌和枯草芽孢桿菌作為微生物指示劑，在不同的臭氧消毒時(shí)間、臭氧濃度、溫度和濕度等條件下，環(huán)境中的菌體殘留量來(lái)檢測(cè)殺菌效果。實(shí)驗(yàn)數(shù)據(jù)表明金黃色葡萄球菌在消毒時(shí)間為2小時(shí)，臭氧濃度在10-15ppm之間，溫度在18-26℃，濕度在60%時(shí)，其檢測(cè)效果比枯草芽孢桿菌靈敏，因此可選用金黃色葡萄球菌對(duì)臭氧消毒后的環(huán)境進(jìn)行檢測(cè)。

關(guān)鍵詞：　生物指示劑 臭氧 消毒

The use of biological indicator of ozone disinfection 

Abstract

Ozone disinfection and sterilization technology for its effective, easy to use, for operators and workplaces no danger, now widely used for the pharmaceutical industry, mainly for the one hundred thousand grade on the environment under the conditions of ozone disinfection, sterilization. In the microbial challenge experiments, the traditional test of ozone disinfection is used to detect Bacillus subtilis, but the results show detection of Bacillus subtilis as an indicator of the existence of more defects, the bactericidal rate was less than a microbial challenge experiments standards. To improve detection sensitivity, were investigated in this experiment in order to Staphylococcus aureus and Bacillus subtilis as an indicator micro-organisms, at different time of the ozone disinfection, ozone concentration, temperature and humidity conditions, the environment to detect the amount of biomass residues bactericidal effect. Experimental data show that the disinfection of Staphylococcus aureus in time is 2 hours, the ozone concentration in the 10-15ppm between the temperature of 18-26 ℃, humidity between 60 ~ 65%, its detection sensitivity better than Bacillus subtilis, it Staphylococcus aureus can be selected after ozone disinfection of environmental testing. 

Key words: aureus subtilis; ozone; microbial challenge test

目錄

摘 要1

Abstract2

第一章 緒論5

1.1臭氧的性質(zhì)5

1.2臭氧消毒原理5

1.3臭氧技術(shù)在制藥工業(yè)上應(yīng)用范圍5

1.4生物指示劑菌種7

1.5本課題的研究目的和意義8

第二章 實(shí)驗(yàn)材料與方法9

2.1 實(shí)驗(yàn)材料與儀器9

2.1.1 主要實(shí)驗(yàn)儀器9

2.1.2 主要實(shí)驗(yàn)試劑9

2.2 實(shí)驗(yàn)方法9

2.2.1實(shí)驗(yàn)菌株9

2.2.2實(shí)驗(yàn)試劑10

2.3 實(shí)驗(yàn)方法10

2.3.1實(shí)驗(yàn)步驟10

2.3.2 菌落的計(jì)數(shù)11

2.3.3 殺菌率11

第三章 結(jié)果與討論12

3.1 消毒時(shí)間對(duì)兩種不同菌種的影響12

3.1.1 消毒時(shí)間對(duì)金黃色葡萄球菌的影響12

3.1.2 消毒時(shí)間對(duì)枯草芽孢桿菌的影響12

3.2 臭氧濃度對(duì)兩種不同菌種的影響13

3.2.1 臭氧濃度對(duì)金黃色葡萄球菌生長(zhǎng)的影響14

3.2.2 臭氧濃度對(duì)枯草芽孢桿菌生長(zhǎng)的影響14

3.3 消毒時(shí)溫度對(duì)兩種不同菌種的影響16

3.3.1溫度對(duì)金黃色葡萄球菌的影響16

3.3.2溫度對(duì)枯草芽孢桿菌的影響16

3.4 消毒時(shí)濕度對(duì)兩種不同菌種的影響17

3.4.1濕度對(duì)金黃色葡萄球菌的影響17

3.4.2濕度對(duì)枯草芽孢桿菌的影響18

第四章 對(duì)檢驗(yàn)殺菌效果的方法達(dá)標(biāo)與否的驗(yàn)證20

4.1金黃色葡萄球菌檢測(cè)條件20

4.2對(duì)金黃色葡萄球菌做微生物指示劑驗(yàn)證20

4.2.1驗(yàn)證方法20

4.2.2浮游菌檢測(cè)20

4.2.3金黃色葡萄球菌檢測(cè)20

4.3殺菌率20

參考文獻(xiàn)22

致謝23