豬繁殖與呼吸綜合征診斷和防制

摘要

豬繁殖與呼吸綜合征（PRRS）近年來在世界范圍內(nèi)迅速蔓延而成為養(yǎng)豬業(yè)的重要威脅。2006年以來，“豬高熱綜合征”肆虐我國養(yǎng)豬業(yè)，高致病性豬藍耳病是其主要致病病原之一。現(xiàn)有疫苗在免疫原性以及交叉保護性方面的作用均值得商榷，此外對于藍耳病病毒的毒力因子的探尋仍無定論。本文主要是通過對PRRS發(fā)現(xiàn)，到研究其致病PRRSV的病原學、流行病學、病理學、實驗室的診斷等諸多方面作一綜述；并在采取諸多防制方面如疫苗免疫、環(huán)境凈化、疫病監(jiān)測的研究做一梳理；并對PRRSV的前景所面臨的問題和困難加以討論。旨在為好的生產(chǎn)環(huán)境提供參考，并為研究PRRS這一領(lǐng)域做鋪墊，最終使人類能快速戰(zhàn)勝這一病種并能合理為目的。現(xiàn)將綜述內(nèi)容簡述如下： 

關(guān)鍵詞　豬繁殖與呼吸綜合征；高致病性豬藍耳病；診斷；防制； 

目錄

摘要  ····························································Ι

目錄  ····························································1

目錄   ···························································2

第一章豬繁殖與呼吸綜合征診斷研究進展  ····························3

1.1 豬繁殖與呼吸綜合征初步診斷  ··································3

1.1.1  PRSSV 病原學   ············································3

1.1.2 流行病學   ·················································4

1.1.3 臨床診斷   ·················································4

1.1.4 病理變化    ················································5

1.1.5 初步診斷小結(jié) ···············································5

1.2 實驗室綜合診斷   ·············································5

1.2.1 病毒的分離與鑒定   ·········································5

1.2.2 血清學實驗   ···············································5

1.2.2.1 免疫過氧化物酶單層細胞實驗（IPMA） ·······················5

1.2.2.2 間接熒光抗體技術(shù)    ······································6

1.2.2.3  ELISA   ·················································6

1.2.2.4  HA和HI 試驗    ·········································6

1.2.3   免疫膠金技術(shù)   ···········································6

1.2.3.1  彩色免疫金銀染色法(IGSS)   ······························6

1.2.3.2  斑點金免疫滲濾測定法   ··································7

1.2.3.3  膠體金免疫層析法   ······································7

1.2.4  分子生物學    ·············································7

1.2.4.1 套式PCR  ················································7

1.2.4.2 多重PCR  ················································7

1.2.4.3 實時熒光定量PCR    ······································7

1.2.4.4  PCR-RFLP    ·············································8

1.2.4.5 核酸探針原位雜交技術(shù)  ····································8

1.2.5   實驗室診斷小結(jié)    ········································8

第二章 豬繁殖與呼吸綜合征防制  ··································10

2.1 疫苗接種    ·················································10

2.1.1弱毒疫苗  ··················································10

2.1.2滅活疫苗   ·············································11

2.1.3核酸疫苗   ·············································11

2.2 凈化  ····················································12

2.3 其它管制  ················································12

小結(jié)與展望    ················································12

參考文獻      ················································13

致謝         ···························