桑葉總黃酮的提取工藝及其抗氧化性的研究

制藥工程論文編號:ZY004    論文字數:11527,頁數:24

目 錄
中文摘要...........................................................I
英文摘要..........................................................II
目錄.............................................................III
1. 緒論............................................................1
1.1抗氧化性物質研究的意義......................................1
1.2 桑葉的簡介......................................... .......3
1.3 黃酮類化合物的抗氧化作用機理...............................3
2. 實驗部分........................................................8
2.1 材料與方法..................................................8
   2.2 黃酮類化合物最佳提取工藝的選擇...............................9
 2.3 桑葉提取物抗氧化性的測定....................................14
3. 結果與討論........................... ..........................17
3.1桑葉中黃酮類提取物的單因素影響. .................................17
 3.2黃酮類化合物影響因素的正交實驗...............................17
 3.3 桑葉提取物抗氧化性研究...................... ...............17
總結與展望..........................................................19
致謝................................................................20
參考文獻.. .........................................................21

摘要：對從桑葉中提取黃酮類化合物的工藝進行了研究．在提取過程中，通過單因素試驗分析了提取液乙醇濃度、提取溫度、提取時間及料液比等4個主要因素對提取率的影響。在單因素的基礎上，通過正交試驗，得到最佳提取工藝，乙醇濃度為60%，料液比為1：30，提取溫度為80℃，提取時間為3 h。對從桑葉提取的黃酮類化合物的抗氧化性，采用還原能力測定和超氧陰離子清除自由基兩種抗氧化性測定方法測定。采用不同的抗氧化性測定模型，測定出來的抗氧化性不同，還原能力測定方法測定出來的抗氧化性要比超氧陰離子清除自由基的方法測定出來的抗氧化性大，并且從中可看出第一個模型的抗氧化性的增大幅度也要大于第二個模型的抗氧化性增大幅度。

關鍵詞：黃酮；提取率；吸光度；抗氧化性

Abstract：The process of extracting flavonoids from Mulberry  is studied．During the extracting process，four main factors including concentration of extracting solution，temperature，time and ratio of solid to liquid are studied by using single factor analysis method．The optimal process conditions are obtained by orthogonal analysis as follows：methanol concentration  60% ，ratio of solid to liquid 1：30，extracting duration 3 h and extracting temperature 80℃ ．Right from the mulberry leaf extract flavonoids oxidation, Reduction in capacity use and removal of super oxide anion radical oxidation resistance of the two measured. Different oxidation resistance of the model, in the oxidation resistance, Reduction Methods for determination of the oxidation resistance than super oxide anion radical removal of the measured antioxidant Large, and can be seen from the first model of the oxidation resistance also increased substantially greater than the second model of a substantial increase oxidation resistance.
Key words：Flavonoids; Extraction rate; Absorbance; Oxidation resistance