對乙酰氨基酚的含量測定及有關(guān)物質(zhì)檢查

制藥工程論文編號:ZY009    論文字數(shù):7563,頁數(shù):21

 目    錄

1.  緒論 …………………………………………………………    1
 1.1  對乙酰氨基酚…………………………………………    1
 1.2  選題背景與意義………………………………………    1
 1.3  含量測定方法…………………………………………    2
2.  實驗部分 ……………………………………………………    4
 2.1  高效液相色譜法………………………………………    4   
 2.2  紫外分光光度法………………………………………    6
3.  結(jié)果與討論 …………………………………………………    7
 3.1  結(jié)果……………………………………………………    7
 3.2  討論……………………………………………………    12
4.  總結(jié)與展望 …………………………………………………    13
致謝 ………………………………………………………………    14
參考文獻 …………………………………………………………    15

摘要：本論文建立對乙酰氨基酚含量測定的方法。采用高效液相色譜法和紫外分光光度法。高效液相色譜法以C18為色譜柱，以甲醇-水 (體積比70∶30)為流動相，流速為1.0 mL／min，檢測波長為250 nm。對乙酰氨基酚在5～30 μg／mL濃度范圍內(nèi)呈現(xiàn)良好的線性關(guān)系(r=0.9997)，回收率為99.6％（RSD=1.9％)。在該色譜條件下對對乙酰氨基酚的雜質(zhì)對氨基酚進行檢查，對氨基酚線性范圍0.1 μg/mL ～1.2 μg/mL。紫外分光光度法測定對乙酰氨基酚含量，結(jié)果表明，對乙酰氨基酚在2～10 ug／mL范圍內(nèi)線性關(guān)系良好，平均回收率為99.9％(RSD=1.1％)。我們建立的兩種含量方法操作簡便，靈敏度高，重復性好，測定結(jié)果準確，可用于對乙酰氨基酚的含量測定。
 關(guān)鍵詞：  對乙酰氨基酚；對氨基酚；高效液相色譜法；紫外分光光度法

Abstract : The purpose of this article was to establish methods for content determination of acetaminophen. We used High-performance liquid chromatography and ultraviolet spectrophotometry. Chromatographic conditions were C18 column and methanol-water (volume ratio 70:30) as the mobile phase. The flow rate was 1.0 mL / min and the detection wavelength was 250nm. In the range of 5～30 μg／mL,  acetaminophen’s concentration showed a good linear relationship (r = 0.9997). The recovery  ratio was 99.6% (RSD = 1.9%). The p-aminophenol  was detected under the same conditions and its linear range was 0.1μg/mL~1.2μg/mL. UV spectrophotometry was used to determine the content of acetaminophen. In the range of 1~10ug/mL, the concentration showed a good linear relationship and the average recovery ratio was 99.9%(RSD = 1.1%). The two methods were simple,  sensitive,  reproducible and accurate, which can be used in the content determination of acetaminophen.

Key words： Acetaminophen；p-Aminophenol ；HPLC； UV spectrophotometry