蟾皮的甲醇提取工藝考察

制藥工程論文編號:ZY014    論文字?jǐn)?shù):6720,頁數(shù):17

目錄
中文摘要 ..........................................................I
英文摘要.......................................................... .II
目錄  ............................ ................................ III
1. 緒論  ........................................................... 1
   1.1 實(shí)驗(yàn)內(nèi)容  ................................................... 1 
2.實(shí)驗(yàn)部分.......................................................... 2
   2.1 實(shí)驗(yàn)試劑、儀器................................................2
   2.2 華蟾酥毒基的含量測定......................................... 2
 2.3  對DM130大孔吸附樹脂柱的考察.............................. 3 
 2.4 提取工藝考察................................................ 4
3. 結(jié)果與討論.......................................................7
   3.1 華蟾酥毒基的含量測定結(jié)果與分析...............................7
   3.2  對DM130大孔吸附樹脂柱的考察結(jié)果與分析................... ..8
 3.3 提取工藝考察的結(jié)果與分析............................. ........9
4. 總結(jié)與展望......................................................11
致謝...............................................................12
參考文獻(xiàn)...........................................................13

摘要：本文詳細(xì)論述了蟾皮的甲醇提取工藝，所用到的試劑、儀器，采用的方法和操作要點(diǎn)。通過文獻(xiàn)考察，知干蟾皮中所含的蟾毒靈、華蟾酥毒基、蟾毒配基等成分均為極性相對比較小的內(nèi)酯類化合物。通過甲醇加熱回流提取與甲醇超聲提取對比實(shí)驗(yàn)，確定出甲醇超聲提取方法。通過對實(shí)驗(yàn)結(jié)果分析，優(yōu)化實(shí)驗(yàn)方案。采用HPLC法以華蟾酥毒基為指標(biāo)對提取液進(jìn)行檢測，流動相對比了藥典方法0.5%磷酸二氫鉀溶液—乙腈流動相系統(tǒng)與文獻(xiàn)方法乙腈—水流動相系統(tǒng)。最終對藥典方法進(jìn)行比例的調(diào)整，即能達(dá)到較好的分離，分離度≥1.5，理論塔板數(shù)按華蟾酥毒基計(jì)不低于2000 。

關(guān)鍵詞：蟾皮；華蟾酥毒基；高效液相色譜法 
Abstract:. This article discusses the process of the methanol extraction of toad skin in detail, the use of reagents and equipments, the methodology and operation of the main points., It is said in literature that Dry skin contains Xenopus Bufalin, cinobufotalin ,China Chansu drug-based, which are lactone compounds with small polarity .. Comparing the heating circumfluence extraction with the ultrasonic extraction of methanol , the latter was chioced in the experiment. Through different tests and the analysis of the experimental results, an optimized experiment  program was determined. Cinobufotalin as the study goals from extract by HPLC .The mobile phase was determind by contrast  the ChP method ( solution of 0.5% potassium dihydrogen phosphate - acetonitrile mobile phase system) with the literature method ( acetonitrile - water mobile phase systems). A better result can be acquired by pharmacopoeia method with  adjusting the ratio of  mobile phase. The resolution is over 1.5, and theoretical plate number in accordance with Cinobufotalin is not less than 2000.
Keywords：Toad skin ; Cinobufotalin; HPLC