關鍵詞：車前子多糖；抗氧化；自由基
Abstract: Antioxidant activity of polysaccharide from Plantain seed was eva luated. Four eva luation methods of antioxidants in vitro such as reducing power, superoxide anion radical scavenging method, hydroxyl free radical scavenging method and DPPH· method was selected among the many experimental models of anti-oxidation. The content of total polysaccharide of the concentrated solution was determined before the study of the antioxident activity. Antioxidant activity study showed that under the experimental conditions of the maximum concentration（0.783962mg/mL）, the plantain seed polysaccharide solution has the maximum scavenging activity in scavenging of superoxide anion , hydroxyl free radical and DPPH free radical. However a maximum antioxidant effect was observed to superoxide anion（O2·-）, followed by DPPH free radical（DPPH·）35.27%, hydroxyl free radical（·OH）16.55%. This study implies that plantain seed has a good antioxidant activity, and provides some basis study in selection the natural antioxidant agents .
