鯊魚軟骨中硫酸軟骨素的提取工藝研究

制藥工程論文編號:ZY095    論文字?jǐn)?shù):11581,頁數(shù):22

目  錄
中文摘要………………………………………………………………………………Ⅰ
英文摘要……………………………………………………………………………Ⅱ
目錄…………………………………………………………………………………Ⅲ
1緒論………………………………………………………………………………1
 1.1引言……………………………………………………………………1
 1.2 硫酸軟骨素的結(jié)構(gòu)與性質(zhì)………………………………………………2
      1.2.1 結(jié)構(gòu)……………………………………………………………………2
      1.2.2理化性質(zhì)………………………………………………………………2
 1.3 鯊軟骨粘多糖的應(yīng)用……………………………………………………2
 1.4 提取路線分析……………………………………………………………3
  1.4.1 酶水解法……………………………………………………………3
  1.4.2 堿提取法……………………………………………………………4
  1.4.3 稀堿和酶結(jié)合法……………………………………………………4
 1.5 提純路線分析………………………………………………………………5
  1.5.1 鹽沉淀法……………………………………………………………5
  1.5.2 利用電離性質(zhì)分離法………………………………………………5
  1.5.3 凝膠過濾色譜分離法………………………………………………5
  1.5.4 電泳分離法…………………………………………………………5
  1.5.5 超濾膜分離法………………………………………………………6
2實驗部分…………………………………………………………………………7
 2.1 原料，試劑與儀器…………………………………………………………7
  2.1.1原料…………………………………………………………………7
  2.1.2試劑…………………………………………………………………7
  2.1.3儀器…………………………………………………………………7
    2.2 實驗流程……………………………………………………………………8
 2.3 實驗方案……………………………………………………………………8

 2.3.1 原料預(yù)處理…………………………………………………………8
 2.3.2 堿解…………………………………………………………………8
 2.3.3 酶解…………………………………………………………………8
 2.3.4 醇析…………………………………………………………………8
 2.3.5 精制…………………………………………………………………9
 2.3.6 產(chǎn)品檢測……………………………………………………………9
3 結(jié)果與討論…………………………………………………………………………10
 3.1 堿解因素對產(chǎn)物的影響……………………………………………………10
 3.2 酶解因素對實驗結(jié)果的影響………………………………………………11
 3.2.1 酶用量的彭響………………………………………………………11
 3.2.2 酶解時間的影響……………………………………………………12
 3.2.3 溫度的影響 ………………………………………………………12
 3.3 三氯乙酸對產(chǎn)物的影響……………………………………………………13
 3.4 醇析條件對產(chǎn)物的影響……………………………………………………14
 3.5 精制條件對產(chǎn)物的影響……………………………………………………14
 3.6 產(chǎn)品表征……………………………………………………………………14
4 總結(jié)與展望………………………………………………………………………16
致謝…………………………………………………………………………………17
參考文獻……………………………………………………………………………18

摘要：以鯊魚軟骨為原料，采用堿液提取，中性蛋白酶水解，三氯乙酸沉淀蛋白酶，乙醇沉淀粘多糖的工藝,提取了硫酸軟骨素。研究了水解鯊魚軟骨的工藝參數(shù)，通過正交實驗獲得中性蛋白酶水解鯊魚軟骨提取硫酸軟骨素的最優(yōu)條件，結(jié)果表明，從鯊魚軟骨中提取硫酸軟骨素的最佳工藝條件為：用質(zhì)量濃度2%的NaOH溶液60oC下提取6h．再用質(zhì)量濃度2%的NaOH溶液提取3h，提取液加人鯊魚軟骨量質(zhì)量分?jǐn)?shù)1.0%的中性蛋白酶，40-50oC水解4h，用三氯乙酸沉淀蛋白質(zhì)，加入4-5倍體積的體積濃度96%的乙醇沉淀，在最佳條件下平均提取率為20.1%。產(chǎn)品為白色粉末。

關(guān)鍵詞：粘多糖；硫酸軟骨素 ；提取；純化

Abstract：In this paper, the Shark cartilage was chosed as the raw material. The technology of the extraction with sodium hydroxide, the hydrolization with dispase, the precipitation of proteinum with trichloroacetic acid and the precipitation of mucoitin with ethanol were mainly studied. The optima conditions of the extraction of chondroitin sulfate were determined by orthogonal experiments  in this paper. The result shows that the most suitable and proper conditions of the extraction of chondroitin sulfate from Shark cartilage were as following : soaked with 2%NaOH for 6 hours at 60oC first, then soaked with 2%NaOH of the first for 3 hours. And then 1.0% dispase were added to the extracts for hydrolization at 40-50oC for 4 hours. The proteinum was precipitated with trichloroacetic acid, and then 4-5 times volume of 96% ethanol were added for the precipitation of mucoitin. The average extraction ratio was 32.2％,and the product was white powder.

Keyword：mucoitin;  chondroitin sulfate;  extraction;  purification