洋蔥油的GCMS分析

制藥工程論文編號:ZY111    論文字數:10376,頁數:26

目  錄
中文摘要 I
英文摘要 II
目錄 III
1. 緒論 1
 1.1 洋蔥的生態習性 1
 1.2 洋蔥的營養成分 1 
 1.3 洋蔥的保健功效 2
 1.4 洋蔥油制備方法 3
 1.5 超臨界萃取原理 3
 1.6 氣相色譜-質譜聯用 5
 1.6.1 氣相色譜 5
 1.6.2 質譜 6
 1.6.3 氣相色譜-質譜聯用 6
2. 實驗部分 7
 2.1 材料與方法 7
 2.1.1 材料和儀器 7
 2.2 洋蔥揮發油的提取 7
 2.3 洋蔥揮發油的分析 8
 2.3.1 氣相分析 8
 2.3.2 氣相色譜-質譜聯用分析 9
3. 結果與討論 9
 3.1 洋蔥油的產率 9
 3.2 洋蔥油的GC-MS分析 10
 3.3 結論 12
4. 總結與展望 12
致謝 13
參考文獻 14
附錄 16

摘 要：本論文主要討論的洋蔥中的活性物質（洋蔥油）及其中各個組分相對含量。考慮到洋蔥油在新鮮洋蔥中的含量極少，故采用脫水洋蔥粉替代，同時洋蔥油中部分為熱敏性物質，為了使其不在萃取過程被破壞分解，決定采用超臨界二氧化碳萃取裝置制備洋蔥油。通過氣相色譜-質譜聯用技術對洋蔥油中化學成分進行了分析，分離出65個色譜峰，鑒定出19個物質，其中有幾個是首次從洋蔥中分離得到的。已鑒定出來的化合物占總峰面積的77.6%，洋蔥油中化合物總數的29.2%。結果顯示，脫水洋蔥粉提取的洋蔥揮發油的主要成分是3, 5-二乙基-1, 2, 4-三硫環戊烷，4-甲基-4-羥基戊酮，6-氨基-1, 3, 5-三嗪基-2, 4-二酚和3, 4-二甲基噻吩等含硫化合物，與文獻中一般提及的以硫醚類化合物為主的洋蔥有所差異。
關鍵詞：洋蔥；超臨界流體；萃取；氣相色譜-質譜聯用

Abstract : This article mainly discuss the active substance (onion oil) in Allium cepa L. and the relative content of various substances. Taking into account the active material in fresh onion in the content is extremely low, we decide to buy dehydraed onion powder instead. In the meanwhile, some components in onion are heat-sensitive materials, in order not to damage those active substance, we finally determine to adopt the preparation method of supercritical carbon dioxide extraction of onion oil. After analyzed by GC/MS, 65 peaks were separated from the oil.and 19 compounds were identified.Some chemicals were the first time separated from the plant.These compounds identified took up 77.6% of the total areas and 29.2% of all compounds.The results showed that main compounds of Chinese Allium cepa L. were the main 3,5-diethyl-1,2,4-trithiane, 4-hydroxy-4-methyl-2-pentanone, 6-amino-1H-
[1,3,5]triazine-2,4-dione, 3,4-dimethylthiophene and other thianes, thiols, sulfides..
These compounds were partly different from these of the same over sea species which had sulfoethers as main components.
Keywords : Onion；Supercritical fluid；Extraction；GC/MS