木霉菌對節瓜枯萎病的生物防治作用

食品科學論文編號:SP021 論文字數:7870,頁數:17

 目    錄
1  前言 1
2  材料與方法 2
2．1  木霉菌的分離純化 2
2．1．1 木霉菌的分離 2
2．1．2 木霉菌的純化和保存 2
2．2  拮抗鐮刀菌的木霉菌篩選 2
2．2．1 供試菌株和材料 2
2．2．2 對峙培養 2
2．3  木霉菌生物學測定 3
2．3．1 菌種來源 3
2．3．2 環境因子對分生孢子萌發的影響 3
2．3．3 環境因子對菌絲生長和產孢的影響 3
3  結果及分析 4
3．1  木霉菌的分離純化 4
3．2  拮抗鐮刀菌的木霉菌篩選 4
3．3  木霉菌生物學測定 6
3．3．1 環境因子對孢子萌發的影響 6
3．3．2 環境因子對菌絲生長和產孢量的影響 7
4  結論與討論 10
4．1  木霉菌的分離純化 10
4．2  拮抗鐮刀菌的木霉菌篩選 10
4．3  木霉菌生物學測定 10
4．3．1  關于環境因子對孢子萌發的影響 10
4．3．2  關于環境因子對木霉菌生長速度和產孢量的影響 11
參 考 文 獻 12
英 文 摘 要 13
致       謝 14

摘    要
    將從土壤中分離出來的8株木霉菌（Trichodema spp.），與節瓜枯萎病原菌進行對峙培養，篩選出拮抗節瓜枯萎病菌鐮刀菌的優良木霉菌T04-004菌株，該菌株對節瓜枯萎病菌的抑制率達90%以上。對生防菌木霉菌T04-004進行生物學特性測定，結果表明，在Richard培養液中，T04-004菌株孢子萌發需要一定的營養，其中以PD培養液最好，并且在PDA培養基上菌絲生長最好，產孢量最大。T04-004菌株孢子萌發，菌絲生長和產孢的溫度范圍為15℃～40℃；T04-004菌株孢子萌發，菌絲生長和產孢的pH值范圍為2～10；光照對菌絲的生長影響不顯著，但對產孢量的影響較大。
關鍵詞：木霉菌 節瓜枯萎病原菌 拮抗作用 生物學特性

 Biological control Effect of Trichodema on Fusarium oxysporum f.sp.benincasae
                     
Abstract：    8 Trichodema were collated from the soil, carries on confronts the Fusarium oxysporum f.sp.benineasae, screens the fine Trichodema T04-004 that oppresses Fusarium oxysporum f.sp.benineasae, This strain to the Fusarium oxysporum f.sp.benineasae 's suppression rate achieved above 90%. The biological characterizations of Trichoderma viride T04-004 were screened and identified. The results illustrated that the spore germination of T04-004 strain needs certain nutrition, and the PD nutrient fluid is the best. The strain T04-004 grows well in the PDA culture medium, including the hypha and spore production. The temperature range of spore sprouts, hypha growth and spore production is 15℃~40℃, and the range of pH-value is 2~10. The illumination is not remarkable influence to hypha growth, but significantly affect on the spore production.
Key words: Trichodema; Fusarium oxysporum f.sp.benincasae; antagonism; biological characteristic