利用微衛(wèi)星引物Bd43A分析桔小實(shí)蠅的多態(tài)性

食品科學(xué)論文編號(hào):SP026 論文字?jǐn)?shù):8236,頁(yè)數(shù):20

目        錄
1  前言 1
2  材料與方法 2
2.1  材料 2
2.1.1  樣品 2
2.1.2  主要儀器設(shè)備和藥品 2
2.2  方法 3
2.2.1  桔小實(shí)蠅成蟲(chóng)采樣 4
2.2.2  試劑盒提取桔小實(shí)蠅總DNA 4
2.2.3  PCR擴(kuò)增 4
2.2.3.1  PCR反應(yīng)引物 4
2.2.3.2  PCR反應(yīng)體系 5
2.2.3.3  PCR反應(yīng)程序 5
2.2.3.4  PCR產(chǎn)物的聚丙烯酰胺凝膠電泳及分析 5
2.3  數(shù)據(jù)記錄和分析 6
3  結(jié)果與分析 6
3.1  微衛(wèi)星引物在桔小實(shí)蠅種群中的擴(kuò)增結(jié)果 6
3.2  桔小實(shí)蠅地理種群的遺傳多樣性 8
3.3  桔小實(shí)蠅地理種群的分化 9
3.4  桔小實(shí)蠅地理種群的聚類(lèi) 10
4  討論 10
參考文獻(xiàn) 12
英文摘要 13
致謝 14
成績(jī)?cè)u(píng)定表及答辯記錄 15

摘    要
   本論文利用微衛(wèi)星標(biāo)記對(duì)四川、云南、廣西、福建四個(gè)地區(qū)桔小實(shí)蠅種群的遺傳多態(tài)性進(jìn)行了分析。通過(guò)提取其基因組DNA,利用微衛(wèi)星引物Bd43A進(jìn)行PCR擴(kuò)增,并進(jìn)行聚丙烯酰胺凝膠電泳，利用Popgene軟件進(jìn)行數(shù)據(jù)分析。結(jié)果表明,這四個(gè)地區(qū)的種群都有較高的遺傳多態(tài)性；福建和廣西、四川和云南的桔小實(shí)蠅種群具有很近的親緣關(guān)系。
關(guān)鍵字  桔小實(shí)蠅  遺傳多態(tài)性  微衛(wèi)星

netic Polymorphism Analysis with Microsatellite Marker Bd43A of Bactrocera dorsalis
Abstract:    By using microsatellite markers, the genetic polymorphism of 4 Bactrocera dorsalis populations collected from provinces of Sichuan, Yunnan, Guangxi and Fujian were analyzed. After extracting the genome DNA, microsatellite primers Bd43A were amplified, and electrophoresed by polyacrylamide gel. Analysed by software Popgene, it was shown that the four Bactrocera dorsalis populations had high genetic polymorphism. The relationships between Fujian and Guangxi, Sichuan and Yunnan were near.
Key words：Bactrocera dorsalis;genetic polymorphism;microsatellite