枯草芽孢桿菌芽孢形成培養(yǎng)條件優(yōu)化

食品科學(xué)論文編號:SP062  論文字?jǐn)?shù):9856,頁數(shù):21

 目  錄
中文摘要 I
英文摘要 II
目錄 III
1. 緒論 1
 1.1  研究目的 1
 1.2研究概況 1 
2. 實(shí)驗(yàn)部分 2
 2.1實(shí)驗(yàn)材料、儀器與方法  2
3. 結(jié)果與討論 4
 3.1培養(yǎng)基成分優(yōu)化    4
 3.2培養(yǎng)條件的優(yōu)化 8
 3.3 枯草芽孢桿菌生長曲線的研究 12
4．總結(jié)與展望 14
 4.1 總結(jié) 14
 4.2 展望 14
致謝 15
參考文獻(xiàn) 16

摘 要：如果無菌灌裝設(shè)備滅菌效果差，飲料在無菌灌裝過程中，可能會(huì)有微生物的滋生，影響產(chǎn)品的品質(zhì)。因此無菌灌裝設(shè)備在出廠前，一般需要無菌驗(yàn)證。枯草芽孢桿菌是一種不易被殺死的細(xì)菌，常用作測試菌。本論文對枯草芽孢桿菌芽孢形成的培養(yǎng)基成分和培養(yǎng)條件進(jìn)行優(yōu)化，為之后無菌灌裝的安全性評價(jià)做了前期準(zhǔn)備。此次試驗(yàn)通過單因素試驗(yàn)，優(yōu)化出ATCC9372菌株最適宜的發(fā)酵培養(yǎng)基組成和培養(yǎng)條件。結(jié)果表明枯草芽孢桿菌的最佳培養(yǎng)基配方為：淀粉5g/L,牛肉膏5g/L ,NaCl 3g/L,K2HPO4 3g/L,MnSO4.H2O 2.4mmol。并將培養(yǎng)基pH調(diào)至7.0，在250mL三角瓶分裝培養(yǎng)基25mL，在37℃下以150r/min 速度搖瓶培養(yǎng)24h。在此培養(yǎng)條件下，枯草芽孢桿菌ATCC9372菌株的活菌數(shù)、芽孢數(shù)和芽孢率均能達(dá)到最高。本實(shí)驗(yàn)還通過采用平板計(jì)數(shù)法和濁度法研究了枯草芽孢桿菌ATCC9372菌株的生長曲線，結(jié)果表明其最佳接種齡為6小時(shí)。

關(guān)鍵詞：枯草芽孢桿菌；芽孢形成；培養(yǎng)基；培養(yǎng)條件
Abstract: Sterilization of aseptic filling equipment, if poor, drinks in aseptic filling process, the microbes may be breeding. So aseptic filling equipment in the factory needs to aseptic validation. Bacillus subtilis is a bacteria that can not easily be killed, often used as test bacteria. The thesis optimized medium components of Bacillus subtilis and culture conditions, For the aseptic filling of the safety eva luation done for it. Through single factor test, optimize the most suitable medium composition and culture conditions. The results showed that Bacillus subtilis optimum medium: starch 5g/L, beef extract 5g/L, NaCl 3g/L, K2HPO4 3g/L, MnSO4.H2O 2.4mmol. And medium pH adjusted to 7.0, in 250mL flask with 10% of the medium ,at 37 ℃ , shaking speed is 150r/min. Under these conditions, viable count, spore count and spore rate to the highest. This study used the plate count and turbidity was studied strains of Bacillus subtilis ATCC9372 growth curve, the results show that the optimal vaccination age of 6 hours.

Keywords：Subtilis; Sporulation; Medium;Culture conditions