乳清小肽免疫調節作用及抗過敏功效初步實驗

食品科學論文編號:SP084  論文字數:10254,頁數:22

目  錄
中文摘要 I
英文摘要 II
目錄 III
1. 緒論 1
 1.1  乳清小肽 2
 1.2實驗目的 2
2. 實驗過程 2
 2.1 試驗目的 2
 2.2受試藥物   2
 2.3試驗動物 2
 2.3.1大鼠 2
 2.3.2小鼠 2
 2.3.3豚鼠 2
 2.4實驗條件 3
 2.5劑量組別 3
 2.5.3給藥途徑與劑量 3
 2.6主要儀器與試劑 3
 2.7實驗方法 5
 2.8觀察指標 8
 2.9統計學分析 8
3. 結果與討論 8
 3.1實驗結果 8
 3.2討論 14
4.結論與展望 15
 4.1結論 15
 4.2展望 15 
致謝 17
參考文獻 18

摘 要：用18-22g的ICR小鼠進行碳粒廓清試驗和血清溶血素測定，用18-22g的BALB/c小鼠進行淋巴細胞增殖反應試驗和NK細胞活性試驗，用180-220g的SD大鼠進行被動皮膚過敏試驗（PCA）。觀察乳清小肽對小鼠碳粒吞噬指數、小鼠血清半數溶血值的影響，觀察乳清小肽對小鼠淋巴細胞增殖反應的刺激指數和NK細胞活性的影響，觀察乳清小肽對被動皮膚過敏大鼠的藍斑面積、藍斑OD值的影響。
 試驗結果表明，乳清小肽對免疫有調節作用；能輕微增強小鼠單核巨噬細胞系統的功能，具有輕微的增強小鼠的非特異性免疫功能的作用，乳清小肽可抑制SRBC免疫小鼠的溶血素抗體的產生，抑制小鼠的特異性體液免疫功能；能抑制小鼠淋巴細胞的增殖反應，抑制小鼠的特異性細胞免疫功能；并且能抑制大鼠被動皮膚過敏反應的作用。
關鍵詞：乳清小肽，碳粒廓清試驗，血清溶血素測定，淋巴細胞增殖反應試驗，NK細胞活性試驗，PCA

Abstract: The experiment is tested with 18-22g of ICR mice carbon clearance test and serum hemolysin, with 18-22g of BALB / c mice lymphocyte proliferation and NK cell activity test, with 180-220g of SD rats were passive cutaneous anaphylaxis test (PCA).Observing the Whey peptides’ impacts on carbon phagocytic index in mice and serum hemolysin.meanwhile,obtaining the results through the  observation of whey peptides on lymphocyte proliferation stimulation index and NK cell activity,and the Observation of whey peptides on the passive cutaneous anaphylaxis in rat locus coeruleus area of OD value of blue spots.
The results show that whey peptides has the effects on the immune regulatory; can slightly enhance the mouse monocyte-macrophage system function, mice with mild non-specific enhancement of immune function,whey peptide can inhibit the immune SRBC hemolysin antibodies, inhibition of specific humoral immune function in mice; can inhibit the proliferation of lymphocytes, inhibition of specific cellular immune function in mice; and could inhibit the role of passive cutaneous anaphylaxis.
Keywords:Whey peptides; Carbon clearance test; Determination of serum hemolysin; Lymphocyte proliferation test; NK cell activity tests; PCA