發酵法生產蝦青素動力學過程研究

食品科學論文編號:SP087  論文字數:14200,頁數:42

摘要:蝦青素是一種有廣闊市場前景的色素，也有抗氧化、抗癌、預防動脈硬化和相關疾病、維護眼睛和中樞神經系統的健康等生物學作用。化學合成蝦青素和甲殼類動物廢棄物的加工有成本高和生產條件要求苛刻等一些缺點。發酵法生產蝦青素是目前成本最低的蝦青素來源（主要是培養基配方價格低廉）。采用了單因素和多因素混合實驗來探討不同氮源、碳源對發夫酵母生產蝦青素的影響。用DNS法測定殘余葡萄糖的量，用干重法測定菌體生物量。結果確定了最佳氮源、碳源。最佳培養基配方為葡萄糖30g/L, （NH4）2SO4 1.5g/L，酵母膏2g/L.種瓶至發酵搖瓶的接種時間為40h，裝液量為50mL。得到了酵母菌的生長曲線,在0-22小時為延滯期，22-49小時進入了指數期，此后為穩定期。

Abstract: Astaxanthin is a kind of pigments that has broad market prospects and has antioxidant; anticancer; Prevention of atherosclerosis; Protect the eyes and central nervous system of health biological Effects. Chemically synthesized Astaxanthin and Crustaceans animal waste processing have some shortcomings that High cost and Production requirements for harsh. Produce Astaxanthin for zymotechnics is the lowest cost way to Produce Astaxanthin（ Is the main medium for the low price） .single factor and mixture design experiments were used to investigate the effects of nitrogen source and carbon source on Phaffia rhodozyma cultivation and astaxanthin production. NDS used measuring residual amount of glucose. Determine optimization of nitrogen sources and carbon source. The best basic medium was composed of 30g/L glucose, 1.5g/L（NH4）2SO4 2g/LYeast Extract. inoculated in 250 ml shake flasks containing 50ml. Gained Yeast growth curve. In 22 hours after inoculation into the exponential phase of growth yeast. 0 -22 hours in the extension of demurrage ,22-49-hour period to enter the index, thereafter stabilizing.
關鍵詞：蝦青素；發夫酵母；碳源；氮源

Keywoeds: Astaxanthin; Phaffia rhodozyma; nitrogen sources; carbon source

目  錄
中文摘要 I
英文摘要 II
目錄 III
1.緒論 1
1.1蝦青素的化學特性 1
1.2蝦青素的生物學功能 1
1.3蝦青素的應用 3
1.4蝦青素的來源 4
1.5課題的研究意義 6
1.6本章小結 6
2.材料與方法 7
2.1 材料 7
2.2試驗方法 7
2.3本章小結 7
3.實驗部分 8
3.1培養基最優氮源的確定 8
3.2培養基最優碳源的確定 8
3.3最佳培養基的確定 9
 3.4發夫酵母生長的動力學 12
3.5本章小結 15
4.實驗總結 16
致謝 17
參考文獻 18