水產(chǎn)品中神經(jīng)性貝類毒軟骨藻酸的高效液相色譜-串聯(lián)質(zhì)譜法測定研究

食品科學(xué)論文編號:SP133  論文字?jǐn)?shù):6021,頁數(shù):15

摘要：本文介紹了對神經(jīng)性貝類毒素研究的背景和意義，對神經(jīng)性貝類毒素的來源其及危害進行了簡單的介紹，并分析了神經(jīng)性貝類毒素的研究現(xiàn)狀，歸納了一些現(xiàn)行的神經(jīng)性貝類毒素的檢測方法，對比了各自的優(yōu)缺點，而高效液相色譜-串聯(lián)質(zhì)譜技術(shù)是目前最優(yōu)的檢測方法，靈敏度高，選擇性好，不易受到其他物質(zhì)的干擾，取得良好的檢測結(jié)果。本文采用LC-MS/MS法對水產(chǎn)品中神經(jīng)性貝類毒素軟骨藻酸含量進行測定，用50%甲醇水溶液提取，通過LC-SAX柱進行凈化，用API3000型色譜-串聯(lián)三重四極桿質(zhì)譜儀分析，結(jié)果滿意。
關(guān)鍵詞：神經(jīng)性貝類毒素;軟骨藻酸;高效液相色譜-串聯(lián)質(zhì)譜

Abstract: This article introduces the right of shellfish toxins on the background and significance of the nerve shellfish poisoning the source of its hazards and a simple presentation and analysis of shellfish poisoning of the status quo, summed up some of the existing Neural shellfish toxin detection methods, the respective comparative advantages and disadvantages, and high-performance liquid chromatography - tandem mass spectrometry technology is the best detection method, high sensitivity and selectivity, susceptible to interference from other substances, Achieve good results. . In this paper, LC-MS/MS method of aquatic products in shellfish toxin domoic acid content was measured, with 50 percent methanol-water solution extraction, LC-SAX-through decontamination, and API3000-chromatography - Series triple quadrupole - Mass spectrometry analysis, with satisfactory results.
Key words: neural shellfish toxin; Domoic acid; high-performance liquid         chromatography - tandem mass spectrometry