食品質(zhì)量分析與檢測

食品科學(xué)論文編號:SP100  論文字?jǐn)?shù):10759,頁數(shù):18

 目  錄 
中文摘要 I
英文摘要 II
目錄 III
1. 緒論 1
 1.1 樣品的制備 1
 1.2 樣品的前處理 2
 1.3  樣品的凈化 3
 1.3.1 氧化鋁固相萃取凈化 3
 1.3.2  凝膠滲透過濾凈化 4
 1.4  高效液相色譜法 4
 1.4.1 高效液相色譜分析原理 4
 1.4.2  高效液相色譜的類型 5
 1.5  色譜分析條件的優(yōu)化 6
 1.5.1 波長的選擇 6
 1.5.2 流動相的選擇 7
 1.5.3 檢測器的選擇 7
 1.5.4 色譜柱、柱溫、流動相流速和進(jìn)樣量的選擇 8
2 實驗部分 9
 2.1 實驗設(shè)備 9
 2.2 實驗試劑與標(biāo)準(zhǔn)品 9
 2.3 色譜條件 9
 2.4 實驗操作 10
 2.4.1 溶劑配制 10
 2.4.2 樣品預(yù)處理 10
 2.4.3 分離原理 10
3 實驗結(jié)果與討論 11
 3.1 檢測波長的選擇 11
 3.2 萃取劑的選擇 11
 3.3 消除色譜干擾的方法 12
 3.4 校正曲線和最低檢出限 14
 3.5 加標(biāo)回收率實驗 14
4 總結(jié)與展望 15
致謝 16
參考文獻(xiàn) 17

摘要：本文評述了高效液相色譜（HPLC）對食品中蘇丹紅檢測的研究進(jìn)展，內(nèi)容著重包括樣品的制備、樣品預(yù)處理、凈化、萃取劑的選擇、檢測波長的選擇、色譜檢測器的選擇等。實驗部分，采用高效液相色譜法測定雞蛋黃中的蘇丹紅Ⅰ、蘇丹紅Ⅱ、蘇丹紅Ⅲ、蘇丹紅Ⅳ。以乙腈提取雞蛋黃中的蘇丹紅，以甲酸、乙腈、丙酮和水按一定變化的比率混合作為流動相，經(jīng)Zorbox-C18色譜柱（4.6mm×150mm）分離，采用478nm，520nm二段變波長檢測，獲得了較好的分離效果和較低的檢測限。該法用于雞蛋中蘇丹紅的測定，蘇丹紅Ⅰ、蘇丹紅Ⅱ、蘇丹紅Ⅲ、蘇丹紅Ⅳ的回收率分別為85%、90%、92%、95%，相對標(biāo)準(zhǔn)偏差分別為2.7%、4.3%、5.3%、3.5%。該法簡便、快速、準(zhǔn)確，能夠滿足實際的檢測需要。
關(guān)鍵詞：食品；檢測；蘇丹紅； 萃取； 高效液相色譜分析

Abstract:  In this paper，the progress on determination of Sudan dyes by HPLC were reviewed. The contents include the pretreatment and purification of sample，the selection of extraction agent of Sudan dyes，wavelength，mobile phase，chromatographic detector，and so on. In the part of experiment， the content of SudanⅠ、SudanⅡ、SudanⅢ、SudanⅣ in chick egg yolk has been determined with a high performance liquid chromatographic method. These Sudan dyes were extracted with acetonitrile. The Sudan dyes were separated on a ZorboxSB-C18 column(4.6mm×150mm)  with a mixture of acetonitrile、acetone、formic acid and water in certain varied ratio as mobile phase. They were then detected at two shifted wavelength 478nm-520nm，with it the experiment gains good effect for separation and low detecting limit. The recoveries of SudanⅠ、SudanⅡ、SudanⅢ、SudanⅣ were 85%、90%、92%、95%  respectively. The relative standard deviations were 2.7%、4.3%、5.3%、3.5% respectively. The method is simple, rapid and accurate，and can meet the needs of practical determination entirely.

Key words: Foodstuff;  Determination;  Sudan Dyes; Extraction;  High Performance Liquid 
           Chromatographic