中藥強化類胡蘿卜素發酵及其產物穩定性研究

生物工程論文編號:SW210  論文字數:12631,頁數:32

目 錄
中文摘要 I
英文摘要 II
目錄 III
1. 緒論 1
 1.1 內容概述 1
 1.2 β-胡蘿卜素和番茄紅素的結構及理化性質 2
 1.2.1 β-胡蘿卜素的結構及理化性質 2
 1.2.2 番茄紅素的結構及理化性質 2
 1.3 β-胡蘿卜素及番茄紅素的生物合成 3
 1.4 β-胡蘿卜素及番茄紅素的生理功能 ..............................4
 1.5 β-胡蘿卜素及番茄紅素的研究意義及現狀  .......................5
2. 實驗部分 5
 2.1 實驗材料...................................................... 5
 2.1.1 菌種.....................................................5
 2.1.2 藥品或試劑...............................................5
 2.1.3 主要儀器.................................................6
 2.1.4培養基................................................... 6
 2.2 實驗方法 .............................................................7
 2.2.1 試驗流程.................................................7
 2.2.2 培養條件.................................................7
 2.2.3 測定方法.................................................7
  2.2.4 中藥提取方法.............................................8
 2.2.5 實驗設計.................................................8
3 .實驗結果和討論 ...................................................10
 3.1不同中藥水提液對三孢布拉氏霉菌（-）生長的影響 ................10
 3.2 標準曲線繪制.................................................16
 3.2.1 β-胡蘿卜素標準曲線繪制................................16
 3.2.2 番茄紅素標準曲線繪制...................................17
 3.3初步篩選出的中藥搖瓶發酵結果..................................18
 3.4各因素對粗提物穩定性的影響....................................18
4．總結與展望 .......................................................26
致謝.................................................................27
參考文獻.............................................................28

 摘要：本論文通過平板培養結合搖瓶發酵，對21種常用中藥進行了大規模篩選，結果表明：在平板上，中藥黃芪，靈芝，枸杞子，太子參對三孢布拉氏霉菌生長有一定的促進作用，中藥三七，川穹，何首烏，桑寄生，丹參，絞股藍，杜仲，陳皮，菟絲子，西洋參，積實，制萸肉對三孢布拉氏霉菌生長有一定的抑制作用，其中三七明顯促進產色素。其它中藥銀杏葉，五味子，大棗，麥冬，野菊花則無明顯的促進及抑制作用。在平板篩選的基礎上，對在平板上有明顯促進作用的中藥進行了搖瓶復篩，結果表明，黃芪對三孢布拉氏霉菌產β-胡蘿卜素、番茄紅素等類胡蘿卜素有一定的提高。接著通過研究溫度，光照，還原劑，氧化劑，酸等因素對粗提物穩定性的影響，表明發酵得到的粗提物應避光冷藏。
 關鍵詞：β-胡蘿卜素；番茄紅素；三孢布拉氏霉菌；中藥

Abstract: Through the combination of plate culture and shake flask fermentation culture, to conduct a large-scale screening with 21 kinds of commonly used Chinese medicine. The results show that  in the plate,Chinese medicine as Astragalus, Ganoderma lucidum, medlar, Pseudostellaria can promote Blakeslea trispora grow well, Chinese medicine as Notoginseng, Chuanqiong, Polygonum multiflorum, Loranthaceae, Danshen, Pentaphyllum，Eucommia, Chenpi, Dodder, American ginseng,Jishi , Zhiyurou have acertain degree of inhibition of growth for Blakeslea trispora, Notoginseng which significantly promote the production of the pigment. Other Chinese medicine ginkgo biloba, schisandrin，Chinese date, Ophiopogon japonicus, wild chrysanthemum have no obvious promotion and inhibition. On the basis of plate culture,have hake flask fermentation culture with the Chinese medicine that have obvious promotion. The results show that Astragalus could enhanced production of β-carotene and lycopene by Blakeslea trispora. Then by studying the temperature, light, reducing agent, oxidizing agents, acids and other factors on the stability of crude extracts from the impact, indicating that fermentation extracts obtained should be frozen and dark.
Keywords：b-carotene; lycopene; Blakeslea trispora; Chinese medicine