分光光度法測定氨基酸型腸內營養劑的膽堿含量

論文編號:SW232  論文字數:9273,頁數:25

分光光度法測定氨基酸型腸內營養劑的膽堿含量
摘  要
 采用分光光度法，在波長500 nm處測定氨基酸型腸內營養劑中的膽堿吸光度。結果表明，在2.5～12.7μg/mL濃度范圍內，吸光度與濃度呈良好的線性關系（r = 0.9997），加標回收率為95.33-104.05%（RSD = 2.8%），方法具有良好的重現性和穩定性，采用該法對日本新引進的氨基酸型腸內營養劑的膽堿含量進行了測定。

關鍵詞：氨基酸型腸內營養劑 膽堿 分光光度法 含量測定

Determination of choline in Enternal Nutrition,Amino Acid,
 Elental by spectrophotometry
ABSTRACT
 In this paper, the absorbance of choline in Enternal Nutrition,Amino Acid, Elental solution was measured by spectrophotometry at the wavelength 500 nm. The results showed that there was a good linear relationship (r = 0.9997) between the absorbance and concentration at the range of 2.5～12.7μg/ml, and the recovery was at the range of 95.33-104.05%（RSD =2.8%）. This method was approved to have good reproducibility and stability which was applied to measure the phosphatidylcholine content in Enternal Nutrition,Amino Acid, Elental which was recommended from Japan.

Key words: Enternal Nutrition,Amino Acid  Elental; lcholine  Spectrophotometry
 Content determination
 

目  錄
      
 摘  要 I
 ABSTRACT II
 1前言 1
 1.1 腸內營養制劑的簡介 1
 1.2膽堿簡介 2
 1.2.1膽堿生理功能，見圖1-2 2
 1.2.2膽堿含量對人體健康的影響 2
 1.3檢測膽堿的目的與意義 3
 1.4不同測定方法的比較與最終選定 4
 2實驗部分 6
 2.1 儀器及試藥 6
 2.1.1 儀器 6
 2.1.2試劑 6
 2.2實驗方法 6
 2.2.1膽堿的定量方法 6
 2.2.1.1原試驗方法[15] 7
 2.2.1.2現擬定方法 7
 3結果與討論 8
 3.1方法學驗證 8
 3.1.1標準曲線的繪制 8
 3.1.2重復性及穩定性 9
 3.1.3顯色時間 10
 3.1.6樣品含量測定 12
 3.2.試驗方法的變更點及其理由 12
 3.2.1供試品溶液制備方法的變更 12
 3.2.2對照品標準溶液制備方法的變更 13
 3.2.3測定條件的變更 14
 3.3總結 14
 3.3.1樣品性質 14
 3.3.2微量含量 14
 3.3.3質量控制 14
 3.3.4溫度控制 15
 4小結 16
 附錄 18
 1.標準曲線制備設計圖 18
 2.回收率計算過程 19
 3.公式 20
 謝辭 21