紅曲霉的原生質體制備研究

論文編號:SW216  論文字數:14416,頁數:22

摘  要：本研究通過一系列單因素試驗（培養基組成、紅曲霉培養時間即菌齡、滲透壓穩定劑種類和濃度、酶系統的組成以及混合酶的濃度、酶解時間等）對紅曲霉原生質體的形成與再生條件進行了研究。結果表明：以豆芽汁培養液為菌絲培養基,采用60h菌齡的菌絲體,以0.6mol/L MgSO4作為滲透壓穩定劑,用1.5%混合酶(蝸牛酶:纖維素酶=7:3) 在30℃下處理2h為最佳的紅曲霉原生質體制備條件，此條件下，原生質體形成數可以達到1.61×107個/ml,再生率為8.49%。
關鍵詞：紅曲霉Ｍ９ｘ；原生質體形成；原生質體再生；最佳條件

Abstract：A series of experiments (including culture medium, the culture time of mycelium, kinds of osmotic stabilizer and its concentration, the proper enzymes and their proportion, enzyme hydrolyzing time) were conducted to study the optimun condition of Monascus rubber protoplast. Results indicated that suitable culture medium was PDA, the culture time of mycelium was 60-day-age, the proper enzymes were snail enzyme and cellulose enzyme, the proper concentration was 1.5% (snail enzyme: cellulose enzyme=7:3), and the osmotic stabilizer was the 0.6mol/L MgSO4. Under the above conditions, the protoplasts formation was 1.61×107/ml, and the regeneration rate was 8.49%.
Key words：Monascus rubber；Protoplasts formation；Protoplasts regeneration；Optimum condition