乳化劑HLB值對莪術油亞微乳理化性質的影響

生物工程論文編號:SW002  論文字數:9937,頁數:19

目  錄
中文摘要 I
英文摘要 II
目錄 III
1. 緒論 1
 1.1 莪述油的基本介紹 1
      1.2 莪述油的提取 1
 1.3 莪述油的化學成分 1
 1.4 莪術油的藥理作用 2
 1.5 莪術油制劑的臨床應用 2
 1.6 莪述油乳劑的制備方法 4
2. 實驗部分 5
 2.1 儀器與藥品 5
 2.2 實驗方法 5
3. 結果與討論 7
 3.1 粒徑和電位 7
    3.2 穩定性 7
 3.3 藥物包封率 8
 3.4 體外釋放 8
 3.5 討論 11
4．總結與展望 12
致謝 14
參考文獻 15

摘要：目的 制備莪術油亞微乳，并考察乳化劑的HLB值對亞微乳理化性質的影響。方法 使用高壓微射流納米分散儀制備含不同HLB值乳化劑的莪術油亞微乳；激光粒度及電泳分析儀測定其粒徑和表面電位；離心法測定其穩定常數；測定藥物在不同HLB值乳化劑的水相中的溶解度，并采用透析法測定亞微乳的包封率和體外釋放。結果制備得到的亞微乳粒徑在123~154 nm 之間，粒子帶負電。幾種乳化劑對藥物均有明顯的增溶作用，增溶倍數從5.5~8.2倍不等，藥物包封率為73～83%，體外釋放符合Higuchi方程，且能持續釋放48小時。結論 乳化劑的HLB值不同，制備得到的亞微乳的性質如粒徑和穩定性有所不同；隨著HLB值增加，乳化劑對藥物的增溶作用增強，藥物包封率略有下降，但體外釋放行為沒有顯著差。

關鍵詞：HLB值；亞微乳；莪術油；穩定性；包封率；體外釋放

Abstract：Objective  To prepare curcuma zedoaria oil-loaded submicron emulsion and investigate the effect of HLB on its physicochemical properties. Methods The high pressure homogenization method was employed to prepare curcuma zedoaria oil-loaded submicron emulsion. The particle distribution and zeta-potential were determined by using zetasizer. The stability was investigated by centrifugalization. The solubilities of zedoaria oil in aqueous phase with various HLB value were determined.  The dialysis method was established for determination of entrapment efficiency and drug release behavior of curcuma zedoaria oil in the submicron emulsion. Results Curcuma zedoaria oil-loaded submicron emulsion was charge negative and with the mean particle size between 123 nm and 154 nm. Solubilizations were observated obviously with emulsifiers of various HLB value, and solubilization multiples were between 5.5  and 8.2. The drug encapsulation efficiencies of curcuma zedoaria oil-loaded submicron emulsion were between 73% and 83%. In vitro drug release experiments indicated the drug release from submicron emulsion was lasted to 48 hours, and the release behavior was consistent with Higuchi equation. Conclusion The curcuma zedoaria oil-loaded submicron emulsions with various HLB value of emulsifier were prepared. Their physicochemical properties such as particle distribution and stability were different accompany with various HLB value of emulsifiers. And as HLB value of emulsifiers increased, more solubilizations were observated, thus entrapment efficiencies were decreased slightly But no significant deviations were observated when released in vitro, and was represented a sustained release.
Key words：HLB value; submicron emulsion; curcuma zedoaria oil; stability; entrapment efficiency; drug release