紫蘇中木犀草素的檢測及提取條件研究

生物工程論文編號:SW007  論文字數:5091,頁數:15

Ⅱ
目  錄
中文摘要 I
英文摘要 II
目錄 III
1. 緒論 1
 1.1  實驗目的 1
 1.2  紫蘇的化學成分 1 
 1.3  木犀草素的藥化作用 2
 1.4  實驗內容簡介 2
2. 實驗部分 3
 2.1  實驗材料與儀器  3
 2.2  實驗步驟 3
3. 實驗結果與討論 5
 3.1  木犀草素的標準曲線  5
 3.2  正交實驗結果 6
 3.3  木犀草素含量的測定 8
4．總結與展望 9
致謝 10
參考文獻 11

摘 要：紫蘇含有多種具有生理活性的化學成分，其中的木犀草素(Luteolin)具有很強的抗氧化性。因此，它可以用于鎮咳,祛痰,平喘,解痙,抗菌,抗腫瘤,抗炎,擴冠 ，其用途非常廣泛。本實驗研究如何從紫蘇中提取木犀草素以及不同部位的紫蘇（葉、梗、籽）和不同地方的紫蘇中木犀草素的含量，采用正交試驗法和高效液相法研究了不同濃度的溶劑和提取方法對于得率的影響，以及不同種類的紫蘇中木犀草素的含量。結果為：乙醇提取法的最優工藝條件確定為：乙醇濃度60%，溫度55℃，顆粒為最小。在最優條件下，木犀草素的最高得率為0.234%。紫蘇籽中木犀草素的含量最高，平均得率達到0.279mg/g。

關鍵詞：木犀草素、紫蘇、正交試驗法、高效液相色譜法

Detection and Extraction Conditions of Luteolin in Perilla

Abstract: Perilla  contained many kinds of  bio-active substances, one of these substances was Luteolin, which had very strong antioxidant ability.Therefore,
it can be used for antitussive,expectorant,asthma,antispasmodic,antibacterial,anti-tumor, anti-inflammatory,dilate coronary,which is widely used.This test is studying how to extract luteolin from perilla,and the contend of luteolin in different parts of perilla (leaves, stems,seeds)and different local of perilla,using orthogonal test method and HPLC to study different concentrations of solvent and extraction method for the yield,as well as different types of basil in the luteolin content. The results are:the optimal process condition of ethanol extraction method to determineare: 60% ethanol concentration,temperature 55℃,the smallest particles. At the optimum conditions,the maximum luteolin yield is 0.234%. Perilla seed in the highest content of luteolin,the average rate is 0. 279mg/g.

Keywords：Luteolin,Perilla,Orthogonal test method,High Performance Liquid
 Chromatography