雷帕霉素發酵條件的優化

生物工程論文編號:SW029  論文字數:6631,頁數:22

目  錄
中文摘要 I
英文摘要 II
目錄 III
緒論 1
 1.1  前言 1
 1.2  雷帕霉素市場現狀 2
實驗部分 4
      2.1  實驗材料 4
      2.2  儀器設備 4
  2.3  實驗方法 4
結果與討論 7
     3.1  斜面培養基的選擇 7
     3.2  種子培養基的選擇  8
     3.3  種子菌齡的選擇 9
      3.4  發酵培養基的優化 11
      3.5  搖瓶收獲時間的選擇 14
      3.6  HAPL萃取條件優化 14
4．總結與展望 16
致謝 17
參考文獻 18

摘  要：本文從雷帕霉素的搖瓶發酵著手，對斜面培養基、種子培養基和發酵培養基的組成和濃度進行優化，同時考察了種子菌齡、搖瓶收獲時間和HPLC萃取條件。實驗主要采用單因素法來比較各因素的影響，以達到優化的目的。雷帕霉素的測定方法有生物活性測定法、薄層層析法和液相色譜法，經過比較得出，高效液相色譜法可以簡潔的定量分析雷帕霉素，而且測定結果比較可靠。在對培養基和各種條件進行優化后，雷帕霉素的產量得到了很大的提高，在優化后的條件下雷帕霉素的產量達到140mg/L，為進一步工業化生產雷帕霉素提供了基礎。   

關鍵詞：雷帕霉素；免疫抑制劑；高效液相色譜；發酵條件優化

Optimization of fermentation conditions for production of Rapamycin

Abstract: In this paper, the rapamycin fermentation to proceed on the slant medium, seed medium and fermentation medium to optimize the composition and concentration, and seed strains studied age, harvest time and shake-flask extraction HPLC conditions. The main experimental single-factor method to compare the impact of various factors in order to achieve the purpose of optimization. Determination of rapamycin has biological activity assay, thin layer chromatography and liquid chromatography, comparison drawn, high-performance liquid chromatography quantitative analysis can be simple rapamycin, and measured results reliable. In the medium and the optimized conditions, the output of rapamycin have been greatly improved in the optimized conditions, the production of rapamycin 140mg / L, for the further industrialization of the production of rapamycin provides the basis.
Keywords：Rapamycin；Immunosuppressant；HPLC；Optimization of fermentation conditions