茯苓多糖提取及氧化研究

生物工程論文編號(hào):SW037  論文字?jǐn)?shù):10290,頁(yè)數(shù):25

目  錄
中文摘要 I
英文摘要 II
目錄 III
1、緒論 1
1.1 茯苓的簡(jiǎn)介 1
1.2 茯苓多糖的結(jié)構(gòu)及作用 1
1.3 茯苓多糖氧化后的作用 2
1.4 茯苓多糖的提取 2
1.5 粗多糖的純化 5
1.6 茯苓多糖的氧化 5
1.7 茯苓多糖及其衍生物體外生物活性的檢測(cè) 6
1.8 實(shí)驗(yàn)思路 6
2、實(shí)驗(yàn)部分 7
2. 材料試劑與設(shè)備 7
2.2 實(shí)驗(yàn)步驟 7
3 結(jié)果與討論 11
3.1 波長(zhǎng)的選擇 11
3.2 標(biāo)準(zhǔn)曲線(xiàn)的制作 11
3.3 單因素分析 12
3.4 茯苓多糖及其氧化衍生物的紅外譜圖 15
3.5 膽酸結(jié)合能力試驗(yàn) 17
4、結(jié)論 18
4.1 結(jié)論 18
4.2 展望 18
致   謝 19
參考文獻(xiàn) 20

 摘 要：以茯苓菌核為原料，在超聲波輔助下，深討了堿溶性茯苓多糖的提取條件。用2，2，6，6-四甲基哌啶-1-氧自由基（TEMPO）將茯苓多糖進(jìn)行氧化，制得氧化茯苓多糖，用紅外光譜進(jìn)行了表征，并用膽酸結(jié)合能力的測(cè)定來(lái)比較多糖及氧化產(chǎn)物的降低膽固醇的能力,結(jié)果表明氧化后的茯苓多糖結(jié)合膽酸的能力比茯苓多糖下降。
 
 關(guān)鍵詞：茯苓多糖；提取；TEMPO；膽酸結(jié)合試驗(yàn)
 
Studies on the Extraction and Oxidation of Poria cocos Polysaccharides

Abstract: Alkali-soluble parchymaran was extracted by using dilute alkali (0.5mol/l NaOH) solution as a solvent with the assistance of ultrasonic and the extraction condition was examined. 2,2,6,6-Tetramethyl-1-piperidine oxoammonium ion (TEMPO)-mediated oxidation was applied to parchymaran. and the physicochemical properties of the resulting derivatives were investigated by using FT-IR and bile acid binding activity. The oxidized derivative showed lower bile acid binding activity than native glucan , it could be explained by the stronger interaction of carboxyl groups and bile acid .
 
Keywords: polysaccharides; extraction; ultrasonic wave; oxidation.