蠶沙葉綠素鐵鈉鹽的制備研究

生物工程論文編號:SW039  論文字?jǐn)?shù):10642,頁數(shù):27

目  錄
中文摘要 I
英文摘要 II
目錄 III
1. 緒論 1
 1.1  前言 1
 1.2  概述 1
 1.3 葉綠素鐵鈉鹽的制備原理 5
 1.4 葉綠素鐵鈉鹽制備的工藝路線 6
 1.5 課題研究的意義 7
2. 實(shí)驗(yàn)部分 8
 2.1 原理、試劑與儀器 8
 2.2 葉綠素提取條件優(yōu)先 9
 2.3 葉綠酸鈉的制備 12
 2.4 葉綠素鐵鈉的制備 14
3. 結(jié)果與討論 16
 3.1 產(chǎn)率與轉(zhuǎn)化率 16
 3.2 吸收曲線的測定 16
 3.2 紅外光譜圖 17
4．總結(jié)與展望 19
致謝 21
參考文獻(xiàn) 22

摘 要: 本論文利用桑蠶的排泄物蠶沙來制取純天然葉綠素鐵鈉，并對其制備制備過程中的提取、皂化和鐵代過程進(jìn)行了最優(yōu)條件的研究。在葉綠素提取工序中，對微波輻射時間、提取溫度、提取時間和提取劑用量做了單因素實(shí)驗(yàn)，結(jié)果表明，在微波處理90s后，用35ml的95%乙醇(相對于5g干蠶沙)在水浴60℃的情況下提取60min為最佳提取條件。在葉綠素皂化工序中，對堿用量、皂化溫度和皂化時間三個因素做了正交試驗(yàn)(糊狀葉綠素與NaOH溶液的體積比為4:1)，結(jié)果表明，用5.0%NaOH溶液在水浴溫度為60℃下皂化50min為最佳皂化條件。在對鐵代工序中，對溶液pH值、溫度和鐵代時間三因素做正交試驗(yàn)(加入的10%FeSO4溶液的體積是所得皂化液體積的四分之一)，結(jié)果表明，鐵代時將皂化溶液的pH值調(diào)為3，水浴溫度為60℃,鐵代處理60min時鐵代反應(yīng)最完全。

關(guān)鍵詞：蠶沙；葉綠素；葉綠素鐵鈉

Study On Preparation Of
Sodium-Iron-Chlorophylln From Silkworm Feces
Abstract: In this thesis, we studied preparation of natural water-soluble Sodium-Iron-Chlorophylln with silkworm excrement from ilkworm. And we studied the optimal conditions of a number of major processes which were extraction, saponification and Iron substitution. In chlorophyll extraction processes, we made Single-factor experiments about some factors which were radiation time, extraction temperature, extraction time, extract dosage. The results showed that the optimum extraction condition was radiation 90s, 60℃, 35ml 95% ethanol(to 5g silkworm excrement as the standard), and 60℃ water bath. In saponification process(the volume ratio of Chlorophyll paste with NaOH solution is 4:1), we made orthogonal about three factors which were base amount, saponification temperature and saponification time. The results showed that the optimum extraction condition is 5.0% NaOH solution, 50min of saponification and 60℃ water bath. In Iron substitution process(the volume ratio of Saponification solution with 10% FeSO4 solution is 4:1), we made orthogonal about three factors which were pH value, temperature and Iron substitution time. The results showed that the optimum extraction condition was pH value 3, 60min of Iron substitution time and 60℃ water bath. 

Keywords: Silkworm excrement; Chlorophyll; Sodium-Iron- Chlorophylln