豬肉中藥物殘留的檢測

生物工程論文編號:SW043  論文字?jǐn)?shù):11751,頁數(shù):29

目  錄
中文摘要 I
英文摘要 II
目  錄 III
1 緒論 1
1.1 克倫特羅 2
1.2 氯霉素 3
1.3 硝基呋喃類藥物 4
1.4 食品檢測的意義和展望 5
2 實驗內(nèi)容 6
2.1 實驗材料 6
2.2 實驗方法 8
3 實驗結(jié)果與討論 15
3.1 各種試劑盒中標(biāo)準(zhǔn)液的標(biāo)準(zhǔn)曲線 15
3.2 實驗結(jié)果 19
3 實驗結(jié)論 22
致  謝 23
參考文獻(xiàn) 24

摘 要：隨著經(jīng)濟(jì)的發(fā)展，膳食結(jié)構(gòu)的不斷改善，動物性食品的需求不斷增加。獸藥在降低動物發(fā)病率與死亡率，提高飼料利用率，促進(jìn)生長和改善產(chǎn)品品質(zhì)等方面起著非常重要的作用。但是，由于管理不當(dāng)和受經(jīng)濟(jì)利益驅(qū)使，各種獸藥被非法大量添加，造成肉類中不同程度的獸藥殘留，對消費(fèi)者的健康產(chǎn)生了各種危害。本論文研究的內(nèi)容為采用的檢測方法為ELISE法，運(yùn)用適當(dāng)?shù)那疤幚恚�再使用試劑盒，通過酶聯(lián)反應(yīng)，對脫水豬肉干進(jìn)行殘留藥物檢測。其主要項目為檢測豬肉中克倫特多、氯霉素、呋喃它酮和呋喃唑酮的殘留量。由于呋喃它酮和呋喃唑酮在體內(nèi)易被代謝，所以在分析此類藥物時采用分析此藥物的代謝產(chǎn)物AMOZ和AOZ。經(jīng)試驗，本實驗所檢驗的20批豬肉樣品中獸藥殘留均符合標(biāo)準(zhǔn)，未出現(xiàn)超標(biāo)現(xiàn)象。

關(guān)鍵詞：豬肉；藥物殘留；酶聯(lián)反應(yīng)；檢測

Study and detection of drug residues in Pork

Abstract:
 With the development of economic and improvement of diet,the d-emand for animal products is increasing.Animal veterinary medicine  have an effect on reducing morbidity,improving feeding efficiency,pr-omoting growth and improving production quality.However,due to mi-smanagement and driven by economic interests,a large number of vet-erinary drugs are added to animal feed,resulting veterinary drug resid-ues with different levels,causing badly health hazards to consumers.T-he most important detection method used in this paper is the ELISE,which by using appropriate pre-processing, react in the Kits,detect thedrug residues of dry pork.The main testing contains the residues of  clenbuterolchloramphenicol,furazolidone and furaltadone.As furaltadoneand furazolidone are susceptible to metabolism,so while dealing with these drugs used in the analysis the metabolites of this drug which  is called AOZ and AMOZ. The results show,these 20 samples of ap-proved veterinary drug residues in pork are standard,the phenomenon of residues more than the standard haven't appeared.

Keywords: Pork;drug residues;ELISE;detection