摘要: 由稻曲病菌（Ustilaginoidea virens）引起的稻曲病，近年來在主要稻區普遍發生，不僅直接影響水稻稻谷的產量，由稻曲病菌產生的毒素也影響著稻米的品質，而且對人們的健康也構成直接的危害，近年來隨著雜交粳稻等感病品種的推廣,土壤肥力水平的提高,致使稻曲病的發生面積及發病程度都有所上升,在某些地區甚至造成嚴重的經濟損失。因此稻曲病已成為稻米糧食安全的重要問題。利用隨機擴增多態性DNA ( random amplified polymorphic DNA , RAPD) 初步分析了稻曲病菌的群體遺傳結構。從81個隨機引物中篩選10個擴增帶型清晰、重復性好的引物, 對采自浙江諸暨、嘉興、武義、金華、桐廬、杭州、富陽、溫州的等水稻種植區的8個菌株進行擴增，10個引物共擴增出84條帶, 引物對來自不同稻區的菌株擴增的DNA 譜型基本相同。但是受限于群體數量，上述結果尚不能明確分析稻曲病菌的遺傳穩定性和時空分布對稻曲病菌變異的影響。但是本實驗探索了采用RAPD研究稻曲病菌群體結構的可用性,為進一步較系統地分析浙江省稻曲病菌系的遺傳變異及群體結構特點奠定基礎。
關鍵詞: 稻曲病; Ustilaginoidea virens; RAPD; 群體結構
Ustilaginoidea virens diversity of the preliminary analysis
Abstract: Rice false smut caused by Ustilaginoidea virens have takenplacegenerally in main rice area in recent years. Rice false smut not only affect the output of paddy, but also influence the quality of the rice because Ustilaginoidea virens can produce toxin. At present reports about the pathogenesis mechanism of rice false smut are rare. In recent years, with the hybrid japonica rice, susceptible variety promotion, raise the level of soil fertility, resulting Rice false smut of size and incidence of disease has increased in some areas and even caused serious economic losses. Thus Rice false smut has become an important rice food security. The genetic diversity and population structure of 81 strains of U. virens collected from different rice were assessed with RAPD (Random ampified polymorphic DNA). A total of 84 bands were amplified with 10 primers from 81 random primers. The preliminary RAPD analysis showed that the genetic diversity of U. virens was not significant. More strains rain s from different fields in different years will be needed in further study to understand the population structure and genetic relation ship of this pathogen in Zhejiang.
Key words: Rice false smut; Ustilaginoidea virens; RAPD; population structure
