利用廉價原料發酵法生產蝦青素

生物工程論文編號:SW057  論文字數:10895,頁數:25

目  錄
中文摘要 I
英文摘要 II
目錄 III
1. 緒論 1
 1.1  蝦青素的結構 1
 1.2  蝦青素的性質 2
 1.3  蝦青素的來源 2
 1.4  蝦青素的功能 3
 1.5 蝦青素的應用 4
 1.6 天然蝦青素的安全性 5
2. 實驗部分 6
 2.1 酵母培養工藝 6
 2.2  實驗材料 6
 2.3  實驗方法 7
3. 結果與討論 8
 3.1  種齡和接種量的選擇 8
 3.2 培養基最優碳源的選擇 9
 3.3 培養基最氮碳源的選擇 11
 3.4  正交設計試驗 13
 3.5  酵母生長曲線的測定 14
4．總結與展望 16
 4.1  總結 16
 4.2  展望 16
致謝 18
參考文獻 19

摘 要：蝦青素是一種酮式類胡蘿卜素，可作為抗氧化劑、色素和食品添加劑等,在醫藥、食品、水產養殖等方面具有非常廣泛的應用。本文概述了蝦青素的結構、性質、來源、功能、應用、發展前景等。本次實驗用紅法夫酵母發酵生產蝦青素，它是天然可產蝦青素的主要微生物之一，研究通過紅法夫酵母發酵法生產得到蝦青素的方法以及生產條件是本次實驗的主要目的。研究方法主要是先通過單因素試驗發現培養基的最佳氮源是尿素，最佳碳源是蔗糖，最佳的接種量是6%。在此基礎上用正交試驗設計法對發酵培養基的成分進行了初步優化，為進一步工業化生產蝦青素提供參考依據。

關鍵詞：紅法夫酵母；蝦青素；廉價原料；發酵

Use of cheap raw materials produced by fermentation of astaxanthin

Abstract: Astaxanthin is a carotenoid keto form can be used as antioxidants, pigments and food additives, medicine, food, aquaculture has a very wide range of applications. This article outlines the structure of astaxanthin, nature, origin, function, application, development prospects. The experimental production of red yeast Phaffia astaxanthin, it is natural astaxanthin can be produced one of the main microorganisms studied by yeast fermentation of Phaffia astaxanthin production by the method and the production conditions of this experiment The main purposes. Research method is to first find the single factor test medium, the best nitrogen source is urea, the best carbon source is sucrose, the best inoculum is 6%. On this basis, orthogonal experimental design method on the fermentation medium components were initially optimized for the industrial production of astaxanthin to further provide a reference. Keywords: Phaffia rhodozyma; astaxanthin; cheap raw materials; fermentation