Fe（Ⅱ）EDTA-NO高效還原菌特性研究

生物工程論文編號:SW082  論文字數:12523,頁數:30

目 錄
中文摘要 I
英文摘要 II
目 錄 III
1. 緒 論 1
1.1  空氣的重要性 1
1.2  NOx的來源及危害 1
1.3  目前的主要治理方法 3
1.4  立題依據及研究內容 4
1.5 小結 5
2. 實驗部分 7
2.1  馴化微生物還原NO絡合吸收液 7
3. 結果與討論 13
3.1  菌種鑒定 13
3.2  氯化銨對于細胞生長和Fe(Ⅱ)EDTA-NO還原效果的影響 13
3.3  不同初始濃度Fe(Ⅱ)EDAT-NO對于還原效果的影響 14
3.4  以Fe(Ⅱ)EDTA和葡萄糖作為電子供體還原Fe(Ⅱ)EDTA-NO時中間產物的測定結果分析 15
3.5  小結 16
4.結論與展望 18
4.1  結論 18
4.2  展望 18
致  謝 19
參考文獻 20
附錄 A 22
附錄 B 24
附錄 C 25

摘 要：本文采用了絡合吸收結合生物轉化的方法，將化學吸收，生物還原法兩種方法結合在一起，首先通過絡合吸收劑的作用，使NO從氣相轉入液相，然后在微生物的作用下將其還原為氮氣，充分利用兩者優勢使其缺陷得以互補。在恒溫搖床中對微生物還原NO絡合吸收液的特性進行了研究。
 實驗結果證明：不同濃度的葡萄糖、Fe(Ⅱ)EDTA-NO、Fe(Ⅲ)EDTA和NH4+ 對DN-3菌還原絡合物Fe(Ⅱ)EDTA-NO能力及其生長有影響。隨著Fe(Ⅱ)EDTA-NO濃度的上升，細菌對其的還原速率先上升后下降，期間達到一個最大比還原速率。加入一定濃度的氯化銨能夠提高Fe(Ⅱ)EDTA-NO的還原速率，但是還原速率增長并不顯著。在外加少量適宜碳源的條件下，就可以實現絡合吸收劑的不斷循環利用。最后鑒定菌種，鑒定結果DN-3菌歸屬為腸桿菌屬中的陰溝腸桿菌(Enterobacter cloacae)。
關鍵詞：絡合吸收；生物還原；細菌分離；鑒定菌種

Fe (Ⅱ) EDTA-NO highly effective return to original state germ characteristic research

Abstract: This article has used the complexing absorption union biotransformation method, The chemical absorption - biology reducing process two methods unifies in together, Through complexing absorbent function, Enable NO to change over to the liquid phase from the gas phase, Then returns to original state under microorganism's function it into the nitrogen, Uses both superiority to enable its flaw fully supplementary. Returned to original state the NO complexing absorption solution characteristic in the constant temperature table to the microorganism to conduct the research.
 Experimental result proof: Different density glucose、Fe(Ⅱ)EDTA-NO、Fe(Ⅲ)EDTA and NH4+ to DN-3 fungus return to original state complex compound Fe(Ⅱ)EDTA-NO The EDTA-NO ability and grows influential. Along with Fe(Ⅱ)EDTA-NO density rise, The bacterium drops after its return to original state fast lead rise, Period achieves a maximum ratio return to original state speed. Joins certain density the ammonium chloride to be able to enhance Fe(Ⅱ)EDTA-NO EDTA-NO return to original state speed, But the return to original state speed grows is not remarkable. In under sur- few suitable carbon source condition, May realize the complexing absorbent unceasing circulation use. Finally appraises the mold mushroom spawn, Appraisal result DN-3 fungus ownership for intestines pole bacteria's in sewer intestines bacillus (Enterobacter cloacae).

Key word: Complexing absorption; Biological return to original state;Bacterium separation;appraises the mold bacterium