摘 要：紅曲色素是一種安全、天然的食品添加劑，具有色價高、毒性低、穩定性好、對蛋白質著色強等優點，被廣泛應用于食品行業中。本研究是通過對實驗室保藏的一株紅曲霉進行紫外誘變，從而獲得了一株紅曲色素產量較高的突變菌株，其發酵色價達到264.00U/g，較出發菌株提高了46.5%。在對色譜的掃描中發現該紅曲紅色素的最大吸收波長為500nm。同時對該突變菌株的發酵條件進行了優化，結果表明接種量為1.5ml，發酵底物為玉米粉，發酵溫度為30℃時，色價最高，達到269.00U/g。并對發酵產物中紅曲色素提取條件進行了研究，結果顯示乙醇濃度為70%，提取時間為60min時提取效果最佳。
關鍵詞：紅曲紅色素；紫外誘變； 發酵條件；提取條件
The breeding of Monascus for producting red pigment
Abstract: Monascus pigment is a safe, natural food additives，and it is widely used in food industry. In order to screening the mutants with higher activities,the mutation breedi- ng of the monascus was carried out by ultraviolet mutagenesis, the mutant strains’s color value is 264.00U/g.Then study the ferment condition of the mutant strain. The results suggested that the fermentable substrates being corn meal and the fermentation temperature being 30℃ are the optimal condition.In this fermentation condition,the color value reach 269.00U/g .Simultaneously, choose the optimum extration process of monascus red pigment.The results suggested that concentration of ethanol being 70% and the extraction time being 60min are the optimal extract condition.
Keywords：Monascus red pigment；Ulltraviolet mutagenesis；Fermentation condition；extration condition
