生物工程論文編號:SW091  論文字?jǐn)?shù):10612,頁數(shù):24

目錄
中文摘要 I
英文摘要 II
目錄 III
1. 緒論 1
 1.1  紅曲色素的組成 1
 1.2  紅曲色素的性質(zhì) 1
 1.3  紅曲色素的生產(chǎn)方法 3
 1.4  紅曲色素的提取方法 4
 1.5   紅曲色素在食品中的應(yīng)用 5
2. 實(shí)驗(yàn)部分 6
 2.1 試驗(yàn)材料 6
 2.2  試驗(yàn)設(shè)計(jì)及方法 6
3. 結(jié)果與討論 10
 3.1   色素的光譜掃描 10
 3.2  紫外誘變   10
 3.3  發(fā)酵條件優(yōu)化 12
 3.4  色素提取工藝的確定 15
4．總結(jié)與展望 17
致謝 18
參考文獻(xiàn) 19

摘 要：紅曲色素是一種安全、天然的食品添加劑，具有色價高、毒性低、穩(wěn)定性好、對蛋白質(zhì)著色強(qiáng)等優(yōu)點(diǎn)，被廣泛應(yīng)用于食品行業(yè)中。本研究是通過對實(shí)驗(yàn)室保藏的一株紅曲霉進(jìn)行紫外誘變，從而獲得了一株紅曲色素產(chǎn)量較高的突變菌株，其發(fā)酵色價達(dá)到264.00U/g，較出發(fā)菌株提高了46.5%。在對色譜的掃描中發(fā)現(xiàn)該紅曲紅色素的最大吸收波長為500nm。同時對該突變菌株的發(fā)酵條件進(jìn)行了優(yōu)化，結(jié)果表明接種量為1.5ml，發(fā)酵底物為玉米粉，發(fā)酵溫度為30℃時，色價最高，達(dá)到269.00U/g。并對發(fā)酵產(chǎn)物中紅曲色素提取條件進(jìn)行了研究，結(jié)果顯示乙醇濃度為70%，提取時間為60min時提取效果最佳。
關(guān)鍵詞：紅曲紅色素；紫外誘變； 發(fā)酵條件；提取條件
The breeding of Monascus for producting red pigment

Abstract: Monascus pigment is a safe, natural food additives，and it is widely used in food industry. In order to screening the mutants with higher activities,the mutation breedi- ng of the monascus was carried out by ultraviolet mutagenesis, the mutant strains’s color value is 264.00U/g.Then study the ferment condition of the mutant strain. The results suggested that the fermentable substrates being corn meal and the fermentation temperature being 30℃ are the optimal condition.In this fermentation condition,the color value reach 269.00U/g .Simultaneously, choose the optimum extration process of monascus red pigment.The results suggested that concentration of ethanol being 70% and the extraction time being 60min are the optimal extract condition.
Keywords：Monascus red pigment；Ulltraviolet mutagenesis；Fermentation condition；extration condition