摘要：建立了高效液相色譜(HPLC) 測定葡萄酒中反式白藜蘆醇方法,并用此方法測定了不同方法釀制的葡萄酒中反式白藜蘆醇的含量，并測定不同酵母菌種、時間、溫度、PH值對釀制葡萄酒中白藜蘆醇含量的影響。實驗中葡萄酒樣品先經過高速離心, 有效地去除了葡萄酒中大量干擾成分。采用Hypersil C18 柱(4.6 mm i.d.×250mm ,5μm) 分離,以乙腈-水為流動相,線性梯度洗脫,用二極管陣列檢測器分別于288 nm 和306 nm 波長下檢測, 白藜蘆醇與其他成分分離良好,提高了分析結果的準確性。采用外標法定量待測組分的質量濃度與其峰面積在一定的范圍內有良好的線性關系,相關系數為0.9973～0.9999。檢測限為0.002～0.005mg/L。對不同方法釀制的葡萄酒的檢測結果表明,白藜蘆醇作為葡萄酒中重要的生物活性成分,其含量與葡萄酒的釀造工藝有密切的關系，不同酵母菌種、時間、溫度、PH值對釀制的葡萄酒中的白藜蘆醇含量產生了影響。該方法還可用于其他天然產物中反式白藜蘆醇含量的測定。
關鍵詞：高效液相色譜;純種發酵;高速離心;反式白藜蘆醇;葡萄酒
The quality analyze of the wine after ferment
ABSTRACT：A method has been developed for the determination of the contents of cis2 and trans-resveratrols and in the wines made by ourselves. by high performance liquid chromatography (HPLC) with fast-divided .The analytes were separated by using a Hypersil C18 column (4.6 mm i. d. ×250 mm , 5μm) and acetonit rile-water as mobile phase with linear gradient elution. A photodiode array detector monitored at the detection wavelen gths of 288 nm and 306 nm for cis-and t r a ns-isomers , respectively. The external standard calibration curves were used in quantitation. There were good linear correlations between the concent rations and peak areas of the four components with the correlation coefficients of 01997 3 - 0. 999 9. The average recoveries were 97.4 %- 98.6 % with relative standard deviations of 2.1 %- 3.2 %. The detection limits were 0.002 – 0.005 mg/ L. The method of HPLC eliminated a great deal of interferences in wines effectively by fast-divided. The effective contents were well separated and the analytical results were improved well and t ruly. The results for determining the wines made by ourselves showed that as an important bioactive substance , the resverat rol content has close relationships with fast-divided methods .The results also showed that it is a convenient , rapid , sensitive and accurate method. It can be used to analyze resverat rols and in other natural samples .
