茶樹菇深層發酵條件的優化

生物工程論文編號:SW102  論文字數:14197,頁數:27

目  錄
中文摘要 Ⅰ
英文摘要 Ⅱ
目錄 Ⅲ
1. 緒論 1
 1.1 食用菌深層發酵研究概況 1
 1.2 真菌多糖的研究概況 3
 1.3 茶樹菇概述 7
2．實驗部分 9
 2.1 實驗材料和設備 9
 2.2 實驗方法 10
3 實驗結果與討論 12
 3.1 碳源對茶樹菇菌絲體干重及胞外粗多糖產量的影響 12
 3.2 氮源對茶樹菇菌絲體干重及胞外粗多糖產量的影響 13
 3.3 兩種無機鹽濃度對茶樹菇菌絲體干重及胞外粗多糖產量的影響 13
 3.4 正交試驗優化培養基組成 14
 3.5 搖瓶裝液量對茶樹菇菌絲體干重及胞外多糖產量的影響 16
 3.6 小結 17
4 總結與展望 17
致　　謝 19
參考文獻 20
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 摘 要:本研究主要對茶樹菇深層發酵培養基和發酵條件的優化,胞外多糖的提取以及其最優發酵條件進行了研究。
  采用搖瓶發酵法研究不同碳源、氮源、無機鹽濃度對茶樹菇菌絲體干重及胞外多糖產量的影響；并用正交試驗獲得了茶樹菇深層發酵的最優培養條件。最優的培養基組成為玉米粉2%、酵母粉0.3%、KH2PO40.2%、MgSO40.05%。同時看到當碳源、氮源濃度再增高時,菌絲體干重和胞外多糖產量都呈下降趨勢,表明高濃度的碳源、氮源對茶樹菇的深層發酵有抑制作用。另外,茶樹菇胞外多糖產量和菌絲生長成一定的正比關系。
 同時還進行了300mL三角瓶的裝液量試驗,得到當裝液量為80mL時,有最大菌絲體干重和胞外多糖產量。
 關鍵詞:茶樹菇；正交試驗；深層發酵；條件優化；裝液量

 Agrocybe cylindracea fermentation conditions of optimization
 Abstract:This research mainly for Agrocybe cylindracea fermentation medium and fermentation conditions optimization, exopolysaccharide extraction and the optimal fermentation conditions.
 This study researchs different carbon sources, nitrogen sources, inorganic salt concentration influences the dry weight of mycelium Agrocybe and Exopolysaccharide Yield by using shake flask fermentation. and orthogonal test was exerted to study Agrocybe cylindracea’s fermentation of optimal condition.And fermentation of optimal condition is: corn meal 2%,dusty yeast 0.3%, KH2PO4 0.2%, MgSO4 0.05%.While when the carbon source, nitrogen concentration increased further, the dry weight of mycelia and exopolysaccharide production both decrease, indicating that high concentrations of carbon and nitrogen sources on the Agrocybe inhibit fermentation. In addition,we can see that Agrocybe Exopolysaccharide production and mycelial growth into a positive relationship.
 At the same time,we have a test of liquid medium volume about 300mL shake flask。We find that the volume of liquid 80mL it have maximum dry weight of mycelium and production of exopolysaccharide.
Keywords：Agrocybe; orthogonal test; submerged fermentation; Optimization; loaded liquid