茶渣水解及產(chǎn)物提取的初步研究

生物工程論文編號(hào):SW109  論文字?jǐn)?shù):10871,頁(yè)數(shù):32

目  錄
中文摘要 I
英文摘要 II
目  錄 III
1  緒論 1
1.1  前言 1
 1.2  茶渣可利用成分 1
 1.3  茶渣的綜合利用 2
 1.4  實(shí)驗(yàn)?zāi)康?nbsp;3
2.實(shí)驗(yàn)部分 4
2.1  實(shí)驗(yàn)材料與試劑 4
2.2  實(shí)驗(yàn)設(shè)備 4
2.3  實(shí)驗(yàn)方法與設(shè)計(jì) 5
2.4  葡萄糖和木糖標(biāo)準(zhǔn)曲線的制作 7
2.5  稀酸水解茶渣的單因素研究 8
3 結(jié)果與討論 9
3.1  料液比對(duì)水解結(jié)果的影響 9
3.2  稀硫酸濃度對(duì)水解結(jié)果的影響 9
3.3  稀硫酸水解溫度對(duì)水解結(jié)果的影響 10
3.4  水解時(shí)間對(duì)水解結(jié)果的影響 11
3.5  響應(yīng)面實(shí)驗(yàn)優(yōu)化水解條件 12
3.6 茶渣脫蛋白結(jié)果與分析 18
3.7 茶渣灰分含量測(cè)定結(jié)果 19
3.8 小結(jié) 19
4 總結(jié)與展望 19
致  謝 21
參考文獻(xiàn) 22
附錄 24

 摘 要：隨著茶產(chǎn)業(yè)的發(fā)展，茶渣大量出現(xiàn)在我們的生活中，面對(duì)如何處理茶渣的問(wèn)題，本文對(duì)稀酸水解茶渣的工藝條件進(jìn)行優(yōu)化。在單因素試驗(yàn)基礎(chǔ)上選取試驗(yàn)因素與水平,根據(jù)Box-Benhnken的中心組合試驗(yàn)設(shè)計(jì)原理對(duì)試驗(yàn)進(jìn)行設(shè)計(jì)和結(jié)果分析。研究了溫度、時(shí)間、稀酸度和料液比對(duì)水解的聯(lián)合影響,得出最佳工藝條件:還原糖水解最佳條件為水解溫度120℃、水解時(shí)間60min、稀酸度3.0%、料液比8.1%。戊糖水解最佳條件為水解溫度120℃、水解時(shí)間60min、稀酸度3.0%、料液比13%。

關(guān)鍵詞：茶渣；水解；響應(yīng)面；提取

A Priliminary Study on hydrolysis and extract the products of Tea Residue

Abstract: With the development of tea industry, there were a large number of tea residue appear in our daily life. The optimize conditions of dilute acid hydrolysis was researched to deal with the problem. On the basis of single factor experiments, the combined effects of temperature, dilute-acid concentration, water - solid ratio were investigated and optimized using RSM. The optimum conditions were as follows: reducing sugar’s are reaction temperature 120℃, reaction time 60min, dilute-acid concentration 3%,water - solid ratio 8.1g/L;pentose’s are reaction temperature 120℃, reaction time 60min, dilute-acid concentration 3%,water - solid ratio 13g/100ml.

Keywords： tea-leaf; hydrolyze; response surface methodology; extract