不同種類殼聚糖抗菌活性研究

生物工程論文編號(hào):SW116  論文字?jǐn)?shù):11546,頁(yè)數(shù):30

目  錄

中文摘要 I
英文摘要 II
目  錄 III
1  緒論 1
1.1  殼聚糖 1
1.2  殼聚糖的主要制備方法 2
1.3  殼聚糖的抗菌性 3
1.4  殼聚糖在其他方面的應(yīng)用 4
1.5  國(guó)內(nèi)外研究狀況 6
2  實(shí)驗(yàn)部分 8
2.1  材料與主要儀器設(shè)備 8
2.2  實(shí)驗(yàn)內(nèi)容 9
3  結(jié)果與討論 13
3.1  經(jīng)測(cè)定篩選出下列五種殼聚糖 13
3.2  CFU的測(cè)定及確定最適稀釋度 13
3.3 抑菌圈的測(cè)定結(jié)果 14
3.4  最低抑菌濃度 17
3.5  自制殼聚糖紅外分析 22
4  總結(jié)與展望 23
致謝 24
參考文獻(xiàn) 25

摘要：殼聚糖具有良好的抗菌活性，將它作為食品防腐抗菌劑的研究具有重大意義。殼聚糖的抗菌作用固然在很大程度上受到本身純度、分子質(zhì)量、脫乙酰度等的影響。本實(shí)驗(yàn)主要對(duì)五種殼聚糖的抗菌性進(jìn)行研究。這五種殼聚糖的分子質(zhì)量及脫乙酰度不同，同時(shí)還對(duì)其在不同濃度下的抗菌性作了相關(guān)探究。得出結(jié)論，CS1對(duì)金黃色葡萄球菌的抗菌作用最好，濃度在0.2g/L可抑制菌的生長(zhǎng)。分子量越大其最低抑菌濃度越低。濃度越高，其抑菌效果越好。因此，對(duì)金黃色葡萄球菌而言，較大分子量的高濃度殼聚糖對(duì)其抗菌效果較顯著。大腸桿菌的抑菌規(guī)律較難歸結(jié)，其抑菌效果一般先隨著殼聚糖分子質(zhì)量的增大而降低，再隨著殼聚糖分子質(zhì)量的增大而增大，CS1對(duì)大腸桿菌的抑菌效果最好。抑菌效果也隨著殼聚糖濃度的增大而增大。殼聚糖對(duì)金黃色葡萄球菌的抑菌效果優(yōu)于大腸桿菌。
關(guān)鍵詞：殼聚糖；抗菌性；分子質(zhì)量；脫乙酰度；濃度

Reach of antibacterial properties on different Chitosan

英文摘要
Abstract: Chitosan has good antibacterial activity, it will have great significance as a food preservative antimicrobial. While antibacterial effect of chitosan are affected by their purity, molecular, degree of deacetylation. Five types of chitosan are studied in this experiment. These chitosan have different molecular, deacetylation degree and antimicrobial. For Staphylococcus aureus, CS1 has the best effect, at a concentration of 0.2g/L can completely inhibit the growth of bacteria. Which with higher molecular, it has the lower minimum inhibitory concentration. The higher the concentration, the inhibitory effect is better. Which Chitosan Inhibition of E. coli is difficult to summary the law. First, its antibacterial activity generally decreased by the molecular of chitosan increasing, again it will increase, CS1 minimum inhibitory concentration is 0.4g/L, it has best effect to inhibited the growth of bacteria. The higher the concentration, the inhibitory effect is better. Chitosan has the antibacterial effect on Staphylococcus aureus is better than E. coli.

Keywords：Chitosan; Antibacterial; Molecular; Deacetylation; Concentration