聚蘋果酸高產(chǎn)菌篩選及發(fā)酵優(yōu)化

生物工程論文編號(hào):SW136  論文字?jǐn)?shù):11256,頁(yè)數(shù):29

目  錄
中文摘要： I
英文摘要：. II
目  錄 III
1  緒論 1
1.1 聚蘋果酸簡(jiǎn)介 1
 1.2  聚蘋果酸應(yīng)用及前景 2
1.3 發(fā)酵法生產(chǎn)聚蘋果酸 6
1.4 課題目的 7
1.5 課題內(nèi)容概述 7
2  實(shí)驗(yàn)內(nèi)容 8
2.1  實(shí)驗(yàn)材料與方法 8
2.2  接種以及篩選菌種 9
2.3  菌落的鑒定與產(chǎn)物測(cè)定 10
3  實(shí)驗(yàn)結(jié)果與討論 11
 3.1  菌種采集 11
 3.2  菌種篩選 11
 3.3  菌落的鑒定與產(chǎn)物測(cè)定 12
3.4  高產(chǎn)菌SP3發(fā)酵條件的研究 16
3.5  聚蘋果酸簡(jiǎn)易測(cè)定法研究 18
4  總結(jié)與展望 20
致  謝 22
參考文獻(xiàn) 24

 摘 要：本論文主要展示了聚蘋果酸的一些特性以及它的一些功能及在各領(lǐng)域中的應(yīng)用。由于人工合成聚蘋果酸步驟復(fù)雜，成本高，因此生物發(fā)酵生產(chǎn)聚蘋果酸就顯得尤為重要了。而本文也主要是設(shè)立方案來(lái)從自然界中篩選出了四種聚蘋果酸產(chǎn)生菌SP1、SP2、SP3、SP4，再?gòu)倪@四種菌株中篩選出了產(chǎn)量最高的菌SP3，并對(duì)其發(fā)酵條件進(jìn)行研究，得出的結(jié)果為其發(fā)酵周期為6-7天，從第8天開(kāi)始將會(huì)產(chǎn)生黑色素，且聚蘋果酸的生成量與菌體量無(wú)關(guān)。同時(shí)也嘗試了一種聚蘋果酸簡(jiǎn)易測(cè)定方法——分光光度法，但是這種方法還需要做進(jìn)一步的研究才有可能適用于聚蘋果酸濃度的測(cè)定。

關(guān)鍵詞：聚蘋果酸；分光光度；發(fā)酵；測(cè)定

Poly malic acid producing bacteria screening and optimization of fermentationAbstract: This article shows some characteristics of poly malic acid , its functions and applications in various fields. As the complexity steps and high cost of synthetic poly malic acid, producing poly malic acid fermentation is especially important. The paper is mainly to establish the program to filter out the poly malic acid producing strains SP1、SP2、SP3、SP4 from the nature, then selected the highest yield of bacteria SP3 from the four strains, and to study the conditions of fermentation, the fermentation period is 6-7 days, then from the 8th day it will have a melanoma, and it is irrelevant between the products of poly malic acid and the biomass. And try an easy method of poly malic acid’s determination - spectrometry, but this method needs further researching, so it can be used in determination of poly malic acid.
Keywords：Poly malic acid; Spectrophotometry; Fermentation; Determination