大蠊中化學成分分離純化研究

生物工程論文編號:SW146  論文字數:11115,頁數:31

目  錄
中文摘要 I
英文摘要 II
目錄 III
1. 緒論 1
 1.1 蟑螂特征 ......................................................1
 1.2 蟑螂真相 .....................................................1
 1.3 藥理作用 .....................................................2
 1.4 分離提取......................................................3
 1.5 化學成分......................................................3
 1.6 實驗目的......................................................4
2. 實驗部分 5
 2.1 實驗材料......................................................5
 2.1.1 實驗樣品................................................5
 2.2 實驗準備工作..................................................5
 2.3 硅膠柱層析....................................................6
 2.4 硅膠薄板層析..................................................7
 2.5 大孔樹脂吸附..................................................7
 2.6 高效液相色譜分析..............................................8
 2.7 LH-20 反相層析................................................8
 2.8 重復實驗......................................................9
 2.9 顯色反應......................................................9
 2.10 液質聯用分析.................................................9
 2.11 合并凍干.....................................................10
3. 結果與討論.........................................................10
 3.1 萃取液的液相圖................................................10
 3.2 KFX-1, KFX-2, K FX-3，KFX-4的分離.............................11
 3.3 KFX-5的分離...................................................15
 3.4 檢測..........................................................18
4．總結與展望.........................................................20
致謝..................................................................22
參考文獻..............................................................23
附錄1(質譜圖) ........................................................25
附錄2(保密協議) ......................................................26

摘要：科學研究者對大蠊進行研究后發現，大蠊中含有的一些化學成分可能有一定得藥物活性。本實驗就是想先用乙酸乙酯對大蠊浸膏進行萃取，除去非極性部分；得到的乙酸乙酯相，用硅膠柱吸附層析，再用高效液相色譜進行分析，然后經LH-20反相層析得到4種目標物質；萃取得到的水相用大孔樹脂吸附，先用20％酒精溶液洗脫除去極性部分，再用40％酒精溶液洗脫收集，然后用高效液相色譜進行分析，再用LH-20反相層析得到1種純的目標物質；最后對分離純化得到的5種目標物質進行顯色反應、紫外光譜及液質等檢測。

關鍵字：色譜；化學成分；大蠊；顯色反應

Studies on the isolation and purification of chemical constituents from Periplaneta

Abstract:
    Scientific researchers studied that Periplaneta contains some of the chemical constituents which may have some a drug ctivity. This experiment firstly want to use ethyl acetate to extract  Periplaneta extraction, remove non-polar part. the phase of ethyl acetate, use silica gel column to carry out adsorption chromatography, and then use high-performance liquid chromatography to analyse,and then by the LH-20 reversed-phase chromatography to get four kinds of target substances; Aqueous extract obtained by macroporous resin adsorption, first with 20% alcohol solution to remove polar elution ,then with 40% alcohol solution elute and collect, and then use high-performance liquid chromatography to analyse, then by the LH-20 reversed-phase chromatography to get one target substance; Finally, the five kinds of target substances during by separating and purificating carry for color reaction, UV and LC-MS detection, etc.

Keywords：Chromatography; chemical constituents; Periplaneta; color reaction