細(xì)菌纖維素高產(chǎn)菌的篩選及培養(yǎng)條件優(yōu)化

生物工程論文編號(hào):SW163  論文字?jǐn)?shù):9950,頁(yè)數(shù):27

目 錄
中文摘要
英文摘要 
目錄
1 緒論 1
1.1 細(xì)菌纖維素的介紹 1
1.2 菌種鑒定 4
1.3 菌種保藏 5
2 實(shí)驗(yàn)部分 5
2.1 實(shí)驗(yàn)材料 5
2.2實(shí)驗(yàn)方法 7
3 結(jié)果與討論 9
3.1 菌株篩選結(jié)果 9
3.2 纖維素檢測(cè) 11
3.3 培養(yǎng)基組成的優(yōu)化實(shí)驗(yàn) 11
3.4 培養(yǎng)方式的確定 14
3.5 菌種初步鑒定 17
4 總結(jié)與展望 20
致謝 21
參考文獻(xiàn) 22

 摘 要：細(xì)菌纖維素是一種新型微生物合成材料，在食品、醫(yī)藥、紡織、化工等方面有著巨大的應(yīng)用潛力。本論文以從小和山市場(chǎng)購(gòu)買的袋裝醋、水果以及XXXXXXXX學(xué)院校內(nèi)采集的土壤為篩選原料，經(jīng)過菌種的富集培養(yǎng),采用稀釋涂布分離法反復(fù)篩選，從久置的醋中分出了一種菌株BV02。該菌株經(jīng)靜態(tài)培養(yǎng)，可產(chǎn)生不溶性的凝膠膜, 通過纖維素酶水解實(shí)驗(yàn)，初步確定其發(fā)酵產(chǎn)物為纖維素。并探討了接種量、種齡、初始pH值和發(fā)酵周期對(duì)細(xì)菌纖維素產(chǎn)量的影響，以確定靜態(tài)培養(yǎng)發(fā)酵生產(chǎn)細(xì)菌纖維素的條件。結(jié)果顯示，菌株BV02的靜態(tài)培養(yǎng)發(fā)酵生產(chǎn)細(xì)菌纖維素的培養(yǎng)條件是：接種量為10％、種齡為24h、發(fā)酵周期7d、發(fā)酵溫度30℃、初始pH值最適范圍是6.0。
 
關(guān)鍵詞：細(xì)菌纖維素；菌株篩選；鑒定；靜態(tài)培養(yǎng)；發(fā)酵條件

Abstract: The bacterial cellulose is widely appllied in food, medicine, textile and chemical industry as a new biosynthetic materia1. This article use some fruit and bagging vinegar brought from market in Xiaoheshan and soil collected in Zhejiang University of Science and Technology as screening material, after enrichment culture and repeated screening by diluting and spreading method, strain BV02 with stable productivity from vinegar was obtained. Through static culture, it can form insoluble gel-like cellulose in the liquid, The main component of gel-like cellulose by BV02 was confirmed to be cellulose. Investigating the fermentation conditions, the results showed that the static fermentation condition of BV02 was 10％ of inoculum size, 24h of seed age, fermentation temperature 30℃, optimal initial pH 6.0 and culture time 7 days.
 
 Key words：Bacterial cellulose; Strain screening; Identification; Static cultivation; Fermentation condition