茯苓多糖的提取及其羥丙基化研究

生物工程論文編號:SW188  論文字數:8548,頁數:21

目錄
中文摘要…………………………………………………………………………Ⅰ
英文摘要…………………………………………………………………………Ⅱ
目錄………………………………………………………………………………Ⅲ  
緒論……………………………………………………………………………1
1.1茯苓多糖的認識…………………………………………………………1
 1.2茯苓多糖提取及修飾的研究進展………………………………………1
 1.3茯苓多糖衍生物…………………………………………………………2
 1.4實驗構想…………………………………………………………………2
2. 實驗部分…………………………………………………………………….3
  2.1  材料與試劑……………………………………………………………3
   2.2 主要儀器設備………………………………………………………….3
 2.3 實驗步驟……………………………………………………………….4
 2.3.1堿溶性茯苓多糖的提取及純化………………………………….4
 2.3.2 多糖含量的測定原理及方法……………………………………6
 2.3.3 茯苓多糖樣品含量測定…………………………………………6
 2.3.4羥丙基衍生物的制備…………………………………………….6
 2.3.5 茯苓多糖的紅外光譜測定………………………………………7
 2.3.6 膽酸結合能力的測定……………………………………………7
 3. 結果與討論………………………………………………………………… 8
   3.1波長的選擇……………………………………………………………  8
   3.2標準曲線的制作…………………………………………………………8
 3.3 茯苓多糖的提取工藝探討…………………………………………… .8
 3.3.1 料液比對茯苓多糖提取率的影響………………………………9
 3.3.2 堿濃度對茯苓多糖提取率的影響………………………………10
 3.3.3 超聲時間對茯苓多糖提取率的影響……………………………11
 3.4紅外光譜分析……………………………………………………………12
3.5 膽酸結合能力評價………………………………………………………13
4.結論與展望……………………………………………………………………15
 4.1結論………………………………………………………………………15
 4.2 展望…………………………………………………………………… 15
致 謝  ………………………………………………………………………… 16
參考文獻…………………………………………………………………………17

 摘要：在超聲波輔助下，采用稀堿提取茯苓多糖，探討了提取工藝，提高了多糖提取率。將堿溶性茯苓多糖進行羥丙基化得到的水溶性羥丙基茯苓多糖，用紅外光譜對其進行了結構表征。通過對膽酸結合能力測試實驗可知，茯苓多糖羥丙基化后能提高多糖對膽酸的結合能力。

關鍵詞：茯苓多糖；提��；超聲波；羥丙基化；膽酸結合能力

Abstract: Alkali-soluble parchymaran was extracted by using dilute alkali (0.5mol/l NaOH) solution as a solvent with the assistance of ultrasonic and the extraction condition was examined. 1.2-epoxypropane-mediated hydroxypropylation was applied to parchymaran, and the physicochemical properties of the resulting derivatives were investigated by using FT-IR and bile acid binding activity. The hydroxypropylated derivative showed higher bile acid binding activity than native glucan .
Keywords:Polysaccharides; Extraction; Ultrasonic wave; Hydroxypropylation
Acid binding capacity.