液相色譜法研究超高效除草劑水解反應動力學

生物工程論文編號:SW199  論文字數:9352,頁數:24

目   錄
中文摘要...................................................................Ⅰ
英文摘要...................................................................Ⅱ
目錄.......................................................................Ⅲ
1  前言..................................................................... 1
 1.1 長殘效除草劑使用概況.................................................. 1
 1.2 嘧啶芐胺類除草劑簡介.................................................. 2
 1.3 新除草劑ZJ0777介紹................................................... 2
 1.4 農藥水解動力學規律.................................................... 3
 1.5 水解速率的影響因素.................................................... 4
 1.6 農藥的水解機理........................................................ 4
 1.7 農藥水解的研究方法.................................................... 5
    1.7.1 預備實驗.......................................................... 5
    1.7.2 不穩定化合物的水解................................................ 5
   1.7.3 水解分析方法...................................................... 6
2  實驗部分 ................................................................ 6
  2.1 主要儀器與試劑........................................................ 6
    2.1.1 儀器.............................................................. 6
    2.1.2 試劑.............................................................. 6
  2.2 實驗準備.............................................................. 6
    2.2.1 實驗器皿的處理.................................................... 6
   2.2.2緩沖溶液的配制 .....................................................7
    2.2.3 ZJ0777儲備溶液的配制...............................................7
    2.2.4 ZJ0777儲備溶液與水的配比實驗.......................................7
  2.3 水解實驗操作.......................................................... 7
  2.4 水解體系的PH檢測......................................................8
  2.5 分析方法.............................................................. 8
    2.5.1 HPLC操作條件的選擇 ............................................... 8
 2.5.2 操作條件.......................................................... 9
 2.5.3 方法回收率........................................................ 9
 2.5.4 方法精密度....................................................... 10
 2.5.5 方法線性關系測定................................................. 10
3  結果與討論.............................................................. 11
 3.1 PH對ZJ0777水解速率的影響............................................ 12
 3.2 溫度對ZJ0777水解速率的影響.......................................... 13
 3.3 ZJ0777水解轉化率..................................................... 15
4  總結與展望.............................................................. 16
致謝....................................................................... 17
參考文獻................................................................... 18

摘要： 本文采用高效液相色譜法研究了新型超高效除草劑N－(2－溴苯基)－2－(4，6－二甲氧基嘧啶－2－基氧)芐胺（ZJ0777）的水解反應。測定的最佳分析條件為： C18反相色譜柱，流動相甲醇:水為0.6：0.4，紫外檢測波長245.8nm，線性范圍5～100mg.L-1，相關系數為0.9998，峰面積變異系數為3.77％，回收率為97.9％～103.6％，檢出限為2.5mg.L-1。研究結果表明：ZJ0777在酸性、中性條件下比較穩定，不易水解，而在堿性條件下隨溫度升高水解速度加快。本研究的目的在于探索ZJ0777的水化學降解行為，作為環境安全性評價的科學依據。

關鍵詞：高效液相色譜；水解；溫度效應；ZJ0777；除草劑

Abstract: The hydrolysis of a highly active herbicide N-(2-bromophenyl)-2- (4,6-dimethoxypyrimidin- 2-yloxy)benzylamine (ZJ0777) in distilled water was studied by HPLC analysis in the paper. RP-HPLC was adopted with C18 column, methanol-water (60:40) as the mobile phase, detecting wavelength was at 240 nm, The calibnation curves were linear with a rang of 5~100 mg. L-1 for ZJ0777, r=0.9998. RSD was 3.77%. Average recovery rate was 97.9~103.6%. The detection limit was 2.5 mg.L-1. The experimental results indicated that ZJ0777 was relative stable in acidic or neutral condition, while the hydrolysis rate increased in basic medium. The study explored the hydrolysis behavior of ZJ0777 as the scientific evidence for environmental eva luation.

Keywords:  HPLC; Hydrolysis; Temperature effect; ZJ0777; Herbicide