一株源于厭氧除磷反應器ＮＬ菌的鑒定及活性研究

論文編號:HJ006  此份論文附開題報告,任務書,論文成績評定表,論文,字數:12064,頁數:28

摘要： 使用從集中水池的泥沙作為接種物，磷酸鹽脫氧磷酸鹽入PH3的還原劑有機體在一臺連續的被攪動的反應器被開化了。 功能細菌通過傳統和現代隔離和證明方法被辨認了。 分類學位置被查明了根據配置的調查、生理和生物化學的物產和16S rDNA分析。 使用絕氧反應器在絕氧情況下，在細菌的基體退化的活動被調查了，表示， NL是一異養的dephosphorization細菌在絕氧情況下。 16S rDNA分析表明張力有98.2%與綠濃桿菌的同源。 它的最佳的碳源是葡萄糖和鈉醋酸鹽，并且最佳的氮氣來源是氨鹽基chloride、nitrate和胨。 然而，纖維素對碳是不合適的。 最佳的最初PH值是6.5～7.0，最佳溫度是30～35 ℃。 當溫度在4 ℃或在35 ℃之上，微生物的活動減少了。 有機磷和鈣的加法有對磷撤除的分明影響，但是還原劑硫化物的加法有沒有對磷撤除的明顯的作用。 關鍵詞： 絕氧dephosphorization; 絕氧dephosphorization細菌; 磷酸鹽deoxidization; 膦

Abstract: Using the silt from concentrate pool as an inoculum, phosphate reducer organisms which deoxidize phosphate into PH3 were cultured in a continuous stirred reactor. The functional bacterium was identified through traditional and modern isolation and identification methods. The taxonomic position was ascertained based on the investigation of configuration, physiological and biochemical properties, and 16S rDNA analysis. The activity in substrate degradation of the bacterium was investigated using the anaerobic reactor under anaerobic condition, which showed that NL is a heterotrophic dephosphorization bacteria under anaerobic condition. The 16S rDNA analysis indicated the strain had a 98.2% of homology with Pseudomonas  aeruginosa. Its best carbon source is glucose and sodium acetate, and the best nitrogen source is ammonium chloride、nitrate and peptone. However, cellulose is unfit for carbon. The best initial pH value is 6.5～7.0, the optimum temperature is 30～35 ℃. The activity of microorganism decreased when the temperature was at 4 ℃ or above 35 ℃. The addition of organic phosphorus and calcium had distinct influence on the phosphorus removal, but the addition of reducer-sulfide had not apparent effect on the phosphorus removal.
Keywords: anaerobic dephosphorization; anaerobic dephosphorization bacteria; phosphate deoxidization; phosphine

目錄
目錄................................................................................ 1
1 前言...............................................................................1
2 材料與方法 ........................................................................3
2.1 使用儀器及設備...................................................................3
2.2 厭氧培養反應裝置.................................................................3
2.2.1 厭氧除磷功能菌培養的混合連續流反應裝置（見圖1） ...............................3
2.2.2 厭氧除磷功能菌株的活性試驗的反應瓶（見圖3）....................................4
2.3 供試材料 ........................................................................5
2.3.1 供試種泥 ......................................................................5
2.3.2 培養基和基礎培養液 ............................................................5
2.4 試驗方法與步驟...................................................................5
2.4.1 厭氧除磷功能菌的培養...........................................................5
2.4.2 厭氧除磷功能菌株的分離、純化與保存.............................................6
2.4.3 顯微形態觀察 ..................................................................6
2.4.4 厭氧除磷功能菌株的生理生化指標試驗[13-15]......................................6
2.4.5 DNA提取、PCR擴增、基因序列比對及進化樹構建 ....................................6
2.4.6 厭氧除磷功能菌株的活性試驗.....................................................6
2.5 指標分析方法 ....................................................................7
2.5.1 總磷、磷酸鹽采用鉬酸銨分光光度法測定[21].......................................7
2.5.2 吸收液磷化氫測定...............................................................8
3 結果與討論.........................................................................8
3.1 NL菌株的形態觀察及生理生化指標...................................................8
3.2 NL菌株的16S rDNA基因的序列、系統發育分析 ........................................9
3.3 NL菌株的活性試驗 ...............................................................10
3.3.1 營養條件對總磷去除及PH3生成的影響.............................................10
3.3.2 環境條件總磷去除及PH3生成的變化過程...........................................16
4 結論 .............................................................................20
參 考 文 獻 ........................................................................21
附    錄 ...........................................................................24
致    謝 ...........................................................................27