殼聚糖-甘油磷酸鈉溫敏凝膠及其緩釋性能研究

材料科學論文編號:CL054  論文字數:11906,頁數:25

目  錄
中文摘要 I
英文摘要 II
目錄
1 緒論 1
 1.1  智能水凝膠在藥物控釋體系方面應用 1
 1.1.1  藥物控釋體系 1
 1.1.2  智能水凝膠載藥體系 2
 1.2  水凝膠概述 2
 1.2.1  pH敏感水凝膠 2
 1.2.2  溫度敏感水凝膠 3
 1.2.3  pH/溫度雙重敏感水凝膠在藥物控制釋放領域的應用 3
2 實驗部分 4
 2.1  實驗試劑和儀器 4
 2.1.1  實驗試劑 4
 2.1.2  實驗儀器 5
 2.2  實驗方法 5
 2.2.1  殼聚糖的檢測 5
 2.2.2  殼聚糖-甘油磷酸鈉溫敏凝膠制備方法 6
 2.2.3  凝膠制備條件的影響因素 6
 2.2.4  凝膠性能測試 7
 2.2.5  載藥凝膠的制備 7
 2.2.6  載藥凝膠的體外藥物釋放 8
3 結果與討論 8
 3.1  殼聚糖粘均分子量的測定 8
 3.2  殼聚糖樣品的脫乙酰度測定 9
 3.3  凝膠化時間的影響因素 10
 3.3.1  GPS/ CS配比對凝膠化時間和溫度影響 11
 3.3.2  pH對凝膠化時間和溫度影響 11
 3.3.3  溫度對凝膠化時間影響 12
 3.4  透光率 12
 3.5  殼聚糖、甘油磷酸鈉樣品及CS-GP凝膠的紅外特征檢測 13
 3.6  載藥凝膠的制備 15
 3.7  載藥凝膠的體外藥物釋放 15
 3.7.1  最大吸收波長的確定 16
 3.7.2  標準曲線的測定 16
 3.7.3  藥物體外累積釋放度的測定 16
4 總結與展望 19
致謝 20
參考文獻 21

摘要：本論文研究制備一種可逆溫敏殼聚糖-甘油磷酸鈉凝膠，并對殼聚糖-甘油磷酸鈉水凝膠的溫敏性能及釋藥行為進行考察。首先對殼聚糖的脫乙酰度以及粘均分子量進行確定，然后通過物理共混法將殼聚糖與甘油磷酸鈉制成半互穿網絡凝膠，研究不同體積配比,不同pH對殼聚糖-甘油磷酸鈉體系凝膠化性能的影響，通過紅外光譜表征殼聚糖-甘油磷酸鈉凝膠；紫外分光光度計測定透光率，考察溫敏凝膠相變曲線。以殼聚糖（質量濃度為2%CS）與甘油磷酸鈉（濃度為56%GPS）的體積比為0.2，調整溶液pH至6.9制備凝膠，以姜黃素為模型藥物，在37℃，40℃的三種PBS緩沖液中測定凝膠的藥物累積釋放度。實驗結果表明：在37℃以上溫度,殼聚糖-甘油磷酸鈉凝膠可快速凝膠化（<260S），姜黃素制成載藥凝膠后，姜黃素的累積釋放度較姜黃素溶液顯著減小。因此該溫敏凝膠可用作藥物的緩釋載體。
關鍵詞：殼聚糖；水凝膠類；姜黃素；緩釋性
 
Abstract: This thesis is to introduce the preparation of reversibly thermosensitive chitosan-glycerophosphate hydrogel, and the investigation of its’ temperature sensitive properties and drug release behaviors. Firstly, the deacetylation degree and the viscosity average molecular weight of chitosan were determined. And then chitosan and glycerophosphate were made into semi-interpenetrating network hydrogel through the physical blending method. The effects of different mass ratio of chitosan to glycerophosphate and different pH on gelation properties of this chitosan-glycerophosphate system were studied. This chitosan-glycerophosphate hydrogel was characterized by infrared absorption spectrometry, and the phase transition curve was obtained through transmittance measured by UV spectrophotometer. Glycerol phosphate(mass concentration 56%)  and chitosan (mass concentration 2% ) in the volume ratio of 0.2, adjusting the solution pH to 6.9 to prepare thermosensitive hydrogel, curcumin as a model drug, the cumulative release rates of  curcumin were measured in three PBS buffer under 37 ℃, 40 ℃. The experimental results show that chitosan-glycerophosphate hydrogel can fast gelled (<260S) when temperature above 37 ℃, and the cumulative release of curcumin hydrogel was significantly lower than that of curcumin solution. Therefore, this temperature sensitive hydrogel can be used as drug sustained release carrier.